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Prior to immunogold-stainings specimens were being incubated for twenty min at 40 to dissolve absent the gelatine and have been then blocked in fifty mM glycine in PBS adopted by blocking in AURION blocking answer (Wageningen, Netherlands)

Coimmunoprecipitation. rabbit anti-KIF21B (Sigma, Taufkirchen, Germany), rabbit unspecific IgG (Dianova, Hamburg, Germany). Western blotting:...