Plasmids encoding FtsZ mutant proteins ended up constructed by internet site-directed mutagenesis of pBAD-FtsZ, pGfp-FtsZ and pEt-FtsZ using the Quik-Site II Mutagenesis kit (Agilent)
A number of other modulators of FtsZ assembly are also not crucial, which includes the FtsZ-associated proteins ZapA, ZapB, ZapC and ZapD . In contrast to the other modulators of…