Blotting. We studied the uptake from the EVs by adipose tissue-derived MSCs (AT-MSCs) making use of confocal microscopy and flow cytometry. We evaluated gene expression utilizing microarrays and assessed osteogenic differentiation markers in AT-MSCs right after culture with the EVs for 18 days.Background: Increasing evidence indicates that mesenchymal stem cell derived extracellular vesicles (MSC-EVs) play a pivotal part in a number of organ repairs. Having said that, their role in cardiovascular regeneration was not nicely studied. The aim was to examine a detailed bioactive content and functional properties of MSC-EVs of various origin in vitro and their regenerative capacity in Caspase 13 Proteins Purity & Documentation Murine model of acute myocardial infarction (AMI) in vivo. Procedures: Murine and human MSCs form bone marrow and umbilical cord tissues, respectively, have been cultured in diverse circumstances including serum-free media. MSC-EVs had been harvested from conditioned media by sequential centrifugation such as ultracentrifugation (100,000 g). MSC-EV morphology and size had been examined by AFM, NTA (Nanosight) and DLS (Izon), the antigen presence- by high-sensitivity FC (Apogee M-50) and WB, the mRNAs/miRNAs content- by real-time RT-PCR, the worldwide proteom -by mass spectrometry. Functional assays in target cardiac and endothelial cells right after iPS-EV therapy in vitro include: proliferation, migration, differentiation, metabolic activity and cell viability analyses. Immunological properties of MSC-EVs have been investigated by way of blood MNC activation in vitro, when regenerative capacity- in murine AMI model in vivo. Results: We discovered MSC-EVs to carry quite a few proteins and mRNA/ microRNA transcripts regulating cardiac and angiogenic differentiation processes. Substantial impact of MSC culture conditions around the molecular and functional properties of MSC-EVs was also confirmed in Glucocorticoid Receptor Proteins Storage & Stability several assays in vitro. Our data also (1) indicated an excellent influence of MSC-EVs on proangiogenic capacity of heart endothelial cells in vitro and (two) confirmed their regenerative prospective in vivo by showing enhanced heart histology, anatomy and function in murine AMI model. Summary/Conclusion: Our information showed that MSC-EVs of distinctive origin represent important carriers transferring bioactive content to mature target cells playing an effective part in heart regeneration in vivo.ISEV 2018 abstract bookWe conclude that MSC-EVs may perhaps represent safe therapeutic tool, alternative or supporting to whole cell-based therapy in cardiovascular repair. Funding: This study was supported by UMO-2013/10/E/NZ3/007500 (NCN) and UMO-2015/16/W/NZ4/00071 (NCN) [grants to EZS]. FBBB JU is often a partner in the Major National Research Center (KNOW) supported by the MSHE.Funding: This study was supported by the grants from National Research Foundation of Korea (NRF) funded by Ministry of Science, ICT Future Arranging [NRF-2017R1C1B2002624], and Convergence Technology Improvement Plan for Bionic Arm by means of the NRF funded by the Ministry of Science, ICT Future Organizing [No. 2017M3C1B2085292].PF03.Regulation of therapeutic compounds in extracellular vesicles by 3Dorganizing distinct physical interactions in between mesenchymal stem cells and culture matrices Sunyoung Jung1; Taehee Kim2; Jinseok Kim1; Hojae Bae3; Oh Young Bang4; Jae Min Cha2 Center for Bionics of Biomedical Study Institute, Korea Institute of Science and Technology, Seoul, Republic of Korea; 2Medical Device Study Center, Research Institute for Future Medicine, Samsung Healthcare Center, Seoul, Republ.
