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Name :
HA tag antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
HA tag Rabbit Polyclonal antibody. Positive WB detected in recombinant protein, Transfected HEK-293 cells. Positive IP detected in Transfected HEK-293 cells. Positive IF detected in Transfected HEK-293 cells.

Tested applications :

Species reactivity :
Recombinant protein; other species not tested.

Alternative names :
CYPYDVPDYA antibody; HA tag antibody

Immunogen :

Isotype :
Rabbit IgG

Preparation :
This antibody was obtained by immunization of Peptide. Purification method: Antigen affinity purified.

Clonality :

Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:2000-1:20000IP: 1:1000-1:10000IF: 1:10-1:100

Background :
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.

References :
Liu Q, Yu J, Zhuo X, Jiang Q, Zhang C. Pericentrin contains five NESs and an NLS essential for its nucleocytoplasmic trafficking during the cell cycle. Cell research. 20(8):948-62. 2010. Liu Z, Luo X, Liu L. Histone acetyltransferase p300 promotes MKL1-mediated transactivation of catechol-O-methyltransferase gene. Acta biochimica et biophysica Sinica. 45(12):1002-10. 2013. Xu LR, Liu XL, Chen J, Liang Y. Protein disulfide isomerase interacts with tau protein and inhibits its fibrillization. PloS one. 8(10):e76657. 2013. Qiu Y, Wang Z, Liu Y. The self-interaction of a nodavirus replicase is enhanced by mitochondrial membrane lipids. PloS one. 9(2):e89628. 2014. Sun X, Lu B, Hu B, Xiao W, Li W, Huang Z. Novel function of the chromosome 7 open reading frame 41 gene to promote leukemic megakaryocyte differentiation by modulating TPA-induced signaling. Blood cancer journal. 4:e198. 2014. Xie HX, Lu JF, Rolhion N. Edwardsiella tarda-Induced cytotoxicity depends on its type III secretion system and flagellin. Infection and immunity. 82(8):3436-45. 2014. Chen Z, Zhu M, Pan X. Inhibition of Hepatitis B virus replication by SAMHD1. Biochemical and biophysical research communications. 450(4):1462-8. 2014. Qiu Y, Miao M, Wang Z. The RNA binding of protein A from Wuhan nodavirus is mediated by mitochondrial membrane lipids. Virology. 462-463:1-13. 2014.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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Author: Gardos- Channel