Name :
HDAC2 antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
HDAC2 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells, Hela cells. Positive IHC detected in human prostate cancer tissue, human breast cancer tissue, human testis tissue. Positive FC detected in HEK-293T cells. Positive WB detected in HEK-293 cells, HeLa cells, HepG2 cells, human kidney tissue, Jurkat cells, MCF7 cells, rat liver tissue. Positive IP detected in mouse testis tissue. Observed molecular weight by Western-blot: 55-60 kDa
Tested applications :
ELISA, WB, IHC, IF, IP, FC
Species reactivity :
Human,Mouse,Rat; other species not tested.
Alternative names :
HD2 antibody; HDAC2 antibody; histone deacetylase 2 antibody; RPD3 antibody; YAF1 antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of HDAC2 recombinant protein (Accession Number: BC031055). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:500-1:5000IP: 1:500-1:5000IHC: 1:20-1:200IF: 1:10-1:100
Background :
Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin.Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). At least 4 classes of HDAC were identified. As a class I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human HDAC2.
References :
Li Z, Hao Q, Luo J. USP4 inhibits p53 and NF-κB through deubiquitinating and stabilizing HDAC2. Oncogene. 2015. Wang X, Liu J, Zhen J. Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy. Kidney international. 86(4):712-25. 2014. Hu X, Lu X, Liu R. Histone cross-talk connects protein phosphatase 1α (PP1α) and histone deacetylase (HDAC) pathways to regulate the functional transition of bromodomain-containing 4 (BRD4) for inducible gene expression. The Journal of biological chemistry. 289(33):23154-67. 2014. Wu S, Ge Y, Huang L, Liu H, Xue Y, Zhao Y. BRG1, the ATPase subunit of SWI/SNF chromatin remodeling complex, interacts with HDAC2 to modulate telomerase expression in human cancer cells. Cell cycle (Georgetown, Tex.). 13(18):2869-78. 2014. Cai M, Hu Z, Liu J. Expression of hMOF in different ovarian tissues and its effects on ovarian cancer prognosis. Oncology reports. 33(2):685-92. 2015.
Related websites: https://www.medchemexpress.com/antibodies.html
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