Share this post on:

Name :
KDM3A,JMJD1A antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
KDM3A,JMJD1A Rabbit Polyclonal antibody. Positive IHC detected in human ovary tumor tissue, human kidney tissue, human ovary tissue. Positive IF detected in HepG2 cells. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, MCF7 cells. Observed molecular weight by Western-blot: 150 kDa

Tested applications :
ELISA, IF, IP, WB, IHC

Species reactivity :
Human, Mouse; other species not tested.

Alternative names :
DKFZp686A24246 antibody; DKFZp686P07111 antibody; JHDM2A antibody; JHMD2A antibody; JMJD1 antibody; JMJD1A antibody; KDM3A antibody; KIAA0742 antibody; lysine (K) demethylase 3A antibody; Lysine specific demethylase 3A antibody; TSGA antibody

Immunogen :
Human KDM3A,JMJD1A Recombinant protein (GST tag)

Isotype :
Rabbit IgG

Preparation :
This antibody was obtained by immunization of KDM3A,JMJD1A recombinant protein (Accession Number: BC038297). Purification method: Antigen affinity purified.

Clonality :
Polyclonal

Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:1000-1:10000IP: 1:500-1:5000IHC: 1:20-1:200IF: 1:20-1:200

Background :
KDM3A, also name as JMJD1A, JHDM2A, JMJD1, JMJD1A, KIAA0742 and TSGA, belongs to the JHDM2 histone demethylase family. It is a histone demethylase that specifically demethylates ‘Lys-9’ of histone H3, thereby playing a central role in histone code. It is involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. It is involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1. (PubMed:16603237), JMJD1A is a useful biomarker for hypoxic tumor cells and a prognostic marker that could be a promising therapeutic target against CRC. (PMID:20823141) This antibody recognize the N-term of KDM3A(JMJD1A).

References :
Uemura M, Yamamoto H, Takemasa I. Jumonji domain containing 1A is a novel prognostic marker for colorectal cancer: in vivo identification from hypoxic tumor cells. Clinical cancer research : an official journal of the American Association for Cancer Research. 16(18):4636-46. 2010. Oh HS, Bryant KF, Nieland TJ. A targeted RNA interference screen reveals novel epigenetic factors that regulate herpesviral gene expression. mBio. 5(1):e01086-13. 2014. Kasioulis I, Syred HM, Tate P. Kdm3a lysine demethylase is an Hsp90 client required for cytoskeletal rearrangements during spermatogenesis. Molecular biology of the cell. 25(8):1216-33. 2014. Ueda J, Ho JC, Lee KL. The hypoxia-inducible epigenetic regulators Jmjd1a and G9a provide a mechanistic link between angiogenesis and tumor growth. Molecular and cellular biology. 34(19):3702-20. 2014. Yamada D, Kobayashi S, Yamamoto H. Role of the hypoxia-related gene, JMJD1A, in hepatocellular carcinoma: clinical impact on recurrence after hepatic resection. Annals of surgical oncology. 19 Suppl 3:S355-64. 2012. Du ZM, Hu LF, Wang HY. Upregulation of MiR-155 in nasopharyngeal carcinoma is partly driven by LMP1 and LMP2A and downregulates a negative prognostic marker JMJD1A. PloS one. 6(4):e19137. 2011. Cho HS, Toyokawa G, Daigo Y. The JmjC domain-containing histone demethylase KDM3A is a positive regulator of the G1/S transition in cancer cells via transcriptional regulation of the HOXA1 gene. International journal of cancer. 131(3):E179-89. 2012. Kim KY, Huerta SB, Izumiya C. Kaposi’s sarcoma-associated herpesvirus (KSHV) latency-associated nuclear antigen regulates the KSHV epigenome by association with the histone demethylase KDM3A. Journal of virology. 87(12):6782-93. 2013.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
Related websites: https://www.medchemexpress.com/antibodies.html
Popular product recommendations:
Anti-Mouse Ly-6G/Ly-6C Antibody (RB6-8C5)
Lebrikizumab
ACAN Antibody: ACAN Antibody is an unconjugated, approximately 208/248 kDa, rabbit-derived, anti-ACAN polyclonal antibody. ACAN Antibody can be used for: ELISA, IHC-P, IHC-F, IF expriments in mouse, rat, and predicted: human, chicken, dog, pig, cow, horse, rabbit background without labeling.

Share this post on:

Author: Gardos- Channel