Name :
TFIIB Protein
Description :
Transcription Initiation Factor IIB (TFIIB) is an essential factor for transcription by RNA Polymerase II. TFIIB localizes to the nucleus where it forms a complex (the DAB complex) with transcription factor IID and IIA. TFIIB plays a role as a bridge between IID, which initially recognizes the promoter sequence, and RNA polymerase II. TFIIB is involved in the selection of transcription start site.
Species :
Human
Uniprotkb :
E. coli
Tag :
N-GST
Synonyms :
TFIIB, GTF2B, TF2B, S300-II, General Transcription Factor TFIIB, Transcription Initiation Factor IIB
Construction :
Recombinant Human Transcription Initiation Factor IIB is produced by our E.coli expression system and the target gene encoding Met1-Leu316 is expressed with a GST tag at the N-terminus.
Protein Purity :
Greater than 81% as determined by reducing SDS-PAGE. (QC verified)
Molecular Weight :
62 KDa, reducing conditions
Endotoxin :
Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Formulatione :
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, pH 8.0.
Reconstitution :
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Stability & Storage :
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.Reconstituted protein solution can be stored at 2-8°C for 2-7 days.Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping :
The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
Research Background :
Transcription Initiation Factor IIB (TFIIB) is an essential factor for transcription by RNA Polymerase II. TFIIB localizes to the nucleus where it forms a complex (the DAB complex) with transcription factor IID and IIA. TFIIB plays a role as a bridge between IID, which initially recognizes the promoter sequence, and RNA polymerase II. TFIIB is involved in the selection of transcription start site.
References and Literature :
Related category websites: https://www.medchemexpress.com/recombinant-proteins.html
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