Activation of Akt/PKB was evaluated by Western Blot on full protein lysates probed with anti-Phospho-Akt Ser473. Extracts ended up also probed with anti-Akt, anti-actin and anti-Xpress (to reveal transfected TBP-one)
We confirmed this observation transfecting U2OS cells with fixed focus of pcDNA-TBP-one and increasing...