Te positions, and up to eleven of these would be required (Table 1). Over the years, our customers have asked us to prepare DNA phosphoramidite mixes for them, and we have provided them as custom products. Now, as the need for degenerate oligonucleotides continues to grow, we have decided to offer these as standard products to cover all eleven possible DNA combinations in equimolar mixes using the most popular versions of our dA, dC and dG. If one would like customized ratios to compensate for different phosphoramidite coupling rates or different protecting groups on the bases, please reach out to our Customer Service Team. All equimolar and customized ratio phosphoramidite mixes will require at least a two-week lead time.
5. Next Generation Sequencing
The arrival of next generation sequencing has allowed researchers to obtain massive sets of data, currently up to billions of reads at a time. Looking through these sizable data sets, one may want to quantify individual oligonucleotides based on the number of reads. However, since most, if not all, sequencing methods require amplification, and such amplification is not uniform, this does not work well. To address this, Unique Molecular Identifiers (UMIs) can be used.9 A 6-12 random nucleotide region is appended to every original strand of template as a molecular barcode to change every original strand into a unique sequence. The UMI, which is sequenced along with the target, becomes part of the data for processing. Instead of counting
Item Mix R: dA+dmf-dG-CE Phosphoramidite Mix Mix Y: Ac-dC+dT-CE Phosphoramidite Mix Mix M: dA+Ac-dC-CE Phosphoramidite Mix Mix K: dmf-dG+dT-CE Phosphoramidite Mix Mix S: Ac-dC+dmf-dG-CE Phosphoramidite Mix Mix W: dA+dT-CE Phosphoramidite Mix Mix H: dA+Ac-dC+dT-CE Phosphoramidite Mix Mix B: Ac-dC+dmf-dG+dT-CE Phosphoramidite Mix Mix V: dA+Ac-dC+dmf-dG-CE Phosphoramidite Mix Mix D: dA+dmf-dG+dT-CE Phosphoramidite Mix Mix N: dA+Ac-dC+dmf-dG+dT-CE Phosphoramidite Mix
In recent years, one type of Click Chemistry called Strain-Promoted Azide-Alkyne Cycloaddition (SPAAC)1 has become particularly popular.865854-05-3 Formula The conjugation reaction is relatively fast, selective, and compatible with biological environments.9028-72-2 supplier The reaction involves an azide and a strained alkyne to form a stable 1,2,3-triazole linkage (Figure 1), and the strained alkyne is the key chemical group that makes it all possible.PMID:30252277 Alkynes are ideally linear, and when they are incorporated into cyclic analogs, particularly smaller ones, the alkyne will have angle strain. While linear alkynes require toxic copper catalysts to facilitate conjugation, strained alkynes will spontaneously react. The formation of the triazole ring converts the higher energy, strained alkyne to an alkene, thereby relieving the ring strain. We offer dibenzocyclooctyne, DBCO, as a modification for facilitating SPAAC with oligonucleotides. There are three phosphoramidites: 5-DBCO-TEG Phosphoramidite, DBCO-dT-CE Phosphoramidite, and DBCO-Serinol Phosphoramidite for 5, internal, and general oligonucleotide labeling, respectively (Figure 2). These products work great. The only caveat to using them is that iodine oxidizer and DBCO are not compatible, which necessitates the use of our mild oxidizing agent, CSO, instead. For a 5 addition, only one cycle of CSO oxidation is required whereas for a 3 or internal insertion, CSO oxidation will be required
throughout the whole synthesis. For those who would like to avoid the use of CSO, we also offer an NHS.MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com
