[email protected] (M.-R.P.) Korean Jindo and Domestic Animals Center, Jindo 58915, Korea; [email protected] (J.-S.K.); [email protected] (S.-J.L.); [email protected] (J.-M.K.) Correspondence: [email protected] (S.-I.O.); [email protected] (B.-H.C.); Tel.: 82-10-4611-5151 (S.I.O.); 82-10-8143-5164 (B.-H.C.) These authors contributed equally.Citation: Sheet, S.; Kim, J.-S.; Ko, M.-J.; Kim, N.-Y.; Lim, Y.-J.; Park, M.-R.; Lee, S.-J.; Kim, J.-M.; Oh, S.-I.; Choi, B.-H. Insight into the Candidate Genes and Enriched Pathways Related with Height, Length, Length to Height Ratio and BodyWeight of Korean Indigenous Breed, Jindo Dog Utilizing Gene Set Enrichment-Based GWAS Analysis. Animals 2021, 11, 3136. https:// doi.org/10.3390/ani11113136 Academic Editor: Maria Teresa Sardina Received: eight September 2021 Accepted: 28 October 2021 Published: two NovemberSimple Summary: The height, length and body-weight are the financial traits of Jindo dogs and it is essential to study their underlying genetic basis. Thus, a post-genome-wide association enrichment study on four body-size traits of Jindo dog which includes height, length, length to height ratio and bodyweight was carried out here. The results revealed a number of possible single nucleotide polymorphisms (SNPs) and genes which had been drastically connected together with the traits of interest. Furthermore, we’ve got found numerous pathways to be drastically linked with the traits of MNITMT Inhibitor interest and these pathways are normally involved in the development and development approach. The findings of this study may well assist to make use of the identified SNPs and genes as possible biomarkers for Jindo breeding. Abstract: As a companion and hunting dog, height, length, length to height ratio (LHR) and bodyweight will be the crucial financial traits for Jindo dog. Human choice and targeted breeding have made an extraordinary diversity in these traits. As a result, the identification of causative markers, genes and pathways that assist us to understand the genetic basis of this variability is essential for their choice purposes. Here, we performed a genome-wide association study (GWAS) combined with enrichment evaluation on 757 dogs employing 118,879 SNPs. The genomic heritability (h2) was 0.33 for height and 0.28 for weight trait in Jindo. At p-value five 10-5 , ten, six, thirteen and eleven SNPs on diverse chromosomes were substantially associated with height, length, LHR and body-weight traits, respectively. Determined by our benefits, HHIP, LCORL and NCAPG for height, IGFI and FGFR3 for length, DLK1 and EFEMP1 for LHR and PTPN2, IGFI and RASAL2 for weight might be the prospective candidate genes as a result of the considerable SNPs positioned in their intronic or upstream regions. The gene-set enrichment analysis IL-4 Protein Data Sheet highlighted right here nine and seven overlapping considerable (p 0.05) gene ontology (GO) terms and pathways among traits. Interestingly, the highlighted pathways were connected to hormone synthesis, secretion and signalling were typically involved inside the metabolism, growth and improvement procedure. Our data deliver an insight in to the considerable genes and pathways if verified additional, that will possess a substantial impact on the breeding from the Jindo dog’s population. Key phrases: indigenous breed; Jindo; height; body-weight; length; GWAS; gene-set enrichment analysis; heritability; hormone synthesis pathwaysPublisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.Copyright: 2021 by the authors. Licensee MDPI,.