O measure the maximum transi scales are are shown in Figure 4. It was observed that the Tmax of collagen from lizardfish scales was shown in Figure four. The DSC curves of collagen from the lizardfish scales scales was the Tmax of collagen from lizardfish tion temperature (Tmax) of collagen. It was observed thatmax values of rat tail collagen, ASC, and PSC were higher than that of rat tail collagen. The T higher than that of rat tail collagen. of collagen from of rat tail collagen, ASC, and PSC The Tmax values lizardfish scales was are shown in Figure 4. It was observed that the Tmax 39.4 , 43.two , and 42.five , respectively. The Tmax of ASC was higher than PSC along with the have been 39.four C, 43.2 C, and 42.5 C, respectively. The Tmax of ASC was higher than PSC and higher than that of rat tail collagen. The Tmax values of rat tail collagen, ASC, and PSC had been rat tail collagen, plus the H of ASC (0.981 J/g) was also larger than the PSC (0.711 J/g) the rat tail collagen, as well as the H of ASC (0.981 J/g) was also higher than the PSC (0.711 J/g) 39.4 , 43.two , and 42.5 , respectively. The Tmax of ASC was higher than PSC as well as the and also the rat tail collagen (0.680 J/g). and the rat tail collagen (0.680 J/g). rat tail collagen, and also the H of ASC (0.981 J/g) was also larger than the PSC (0.711 J/g) along with the rat tail collagen (0.680 J/g).2.6. Thermal StabilityFigure four. The DSC curve of ASC and PSC. The experiment was performed only as soon as (n = 1). Figure four. The DSC curve of ASC and PSC. The experiment was performed only once (n = 1).two.7. Solubility 2.7.1. of ASC and PSC. The experiment was performed only when (n = 1). Figure 4. The DSC curveThe Influence of pH on the Solubility of Collagen Solutions The relative solubility of ASC and PSC extracted from the scale of lizardfish at PF-06873600 Biological Activity various pH showed equivalent trends, as shown in Figure 5a. ASC and PSC exhibited higher relative solubility within the quite acidic pH variety (1), and each ASC and PSC showed the maximum relative solubility at pH 2. The relative solubility of ASC and PSC decreased with growing pH, plus a sharp reduce in relative solubility of ASC and PSC occurred at pH above five and 4, and the minimum relative solubility was 11.09 and 7.70 , respectively. The isoelectric points (pI) of ASC and PSC had been around around 7 and 8, VBIT-4 Purity respectively [33].Mar. Drugs 2021, 19,ardfish scales is shown in Figure 5b. ASC and PSC showed higher relative solubility at low NaCl concentrations, each above 80 . The relative solubility of ASC and PSC from lizard fish scales decreased with rising NaCl concentrations, using the lowest values at 14 and 10 and 11.42 and 13.64 NaCl concentrations, respectively. Subsequently, as the 7 of 17 NaCl concentration elevated, the relative solubility of collagen remained fairly stable but extremely low (around 20 ).(a) (b)Figure five. Relative solubility of ASC and PSC. (a) Effect of pH; (b) effect of NaCl concentration. Val Figure 5. Relative solubility of ASC and PSC. (a) Effect of pH; (b) effect of NaCl concentration. Values ues represent the means common deviations (SD) of duplicate assays (n = 3). Unique letters in represent the signifies normal deviations (SD) of duplicate assays (n = 3). Diverse letters indicated dicated substantial differences among the samples. considerable differences in between the samples.2.7.2. The Influence of NaCl two.eight. Rheological Properties Concentration on the Solubility of Collagen Options The impact of NaCl concentration around the re.
