Se (PCS) (c44643), natural resistanceassociated macrophage protein 3 (NRAMP3) (c48489), Heavy metal RGS19 Molecular Weight ATPase three (HMA3) (c48855), and Heavy metal ATPase 5 (HMA5) (c64219) (Table five). NAS can catalyze the formation of nicotianamine (NA) from S-adenosylmethionine (SAM). As a essential element of metal ions homeostasis in plants, NA was a metal ion chelator, which can chelate divalent metal ions, for instance Mn2+, Zn2+, Cu2+, Fe2+, etc. (Clemens et al. 2013). When the hyperaccumulator plant S. alfredii was treated with CdCell wall immobilizationThrough comparative transcriptome analysis of P. americana in response to Cd stress, there have been 3 KEGG pathwaysTable 5 DGEs associated with heavy metal accumulation and tolerance genes in P. americanaSpecific function 0 h HMA3 belongs to P1B-type ATPase subfamily and exhibit Cd-specific transport activity HMA5 participates Cu compartmentalization and detoxification MT3 has a higher content material of Cysc. MT3 is associated with heavy metal detoxication, and ROSd scavenging K05941 K12347 K14692 K05953 K14709 K14709 K00640 K00789 K00549 K00799 c65306 c65693 c61336 c65540 c63408 c72366 c70912 c54726 c56713 c68822 c71425 c72656 c61692 c48489 1 1 1 1 1 1 1 1 1 1 1 1 1 1 c44643 1 1.06 two.03 two.31 2.28 0.64 0.45 four.47 1.00 two.12 1.58 three.06 two.11 two.36 1.84 2.56 c29649 1 1.68 K17686 c64219 1 five.68 9.11 2.31 1.43 2.85 two.22 1.04 0.27 0.47 four.27 0.45 0.60 0.46 37.07 1.28 1.27 1.40 three.34 K01534 c48855 1 2.40 4.60 1.89 21.87 1.44 0.97 2.12 3.45 3.00 0.38 0.66 6.43 1.07 1.21 1.07 61.67 two.41 1.00 11.41 4.93 two h 12 h 24 h Up Up Up Up Up Up Up Down Down Up Up Up Up Up Up Up Up Up KO No. a DEG unigene Fold changeb RegulationPage 18 ofGene nameHeavy metal ATPase three (HMA3)PCS plays a essential role in the tolerance of plants to heavy metal via the synthesis of PCs, which can chelate heavy metal in vacuole and then transport mTORC1 custom synthesis towards the vacuole NRAMP3 locates around the membrane of tonoplast and NRAMP3 can transport a range of heavy metals to vacuole MTP is divalent cation transporter and plays a vital part in plant heavy metal tolerance NAS is really a crucial component of plant metal homeostasis via the synthesis of NA, which can chelate divalent heavy metal ions ZNT1 is actually a plasma membrane transporter which will transport Zn but not Cd, Fe, Mn, or Cu into plant cells ZNT4 may locate in chloroplast and takes aspect within the transport of Zn inside the plastids SAT1 is often a important enzyme inside the biosynthesis of Cys in plants and SAT is involved in plant sulfur metabolism MAT catalyzes the production of Exact same and participates in Metf metabolism. SAM is the active methyl donor MetE catalyzes the formation of Met through transferring the methyl group type tetrahydrofolate (Ne-CH3-FH4) to Hcy g, which is the final step of Met biosynthesis GST is involved in heavy metal detoxification via conjugating GSHh to heavy metal. GST can enhance plant tolerance to heavy metal anxiety by enhancing the antioxidant program in plantsHeavy metal ATPase five (HMA5)Metallothionein-like protein kind three (MT3)Phytochelatin synthase (PCS)Organic resistance-associated macrophage protein three (NRAMP3)Metal tolerance protein (MTP)Nicotianamine synthase (NAS)Zn transporter 1 (ZNT1)Zn transporter four (ZNT4)Serine acetyltransferase 1 (SAT1)S-adenosylmethionine synthase (MAT)Cobalamin-independent methionine synthase (MetE)Glutathione S-transferase (GST)abKEGG Orthology (KO) entrycFold change in comparison with FPKM value at 0 hdCys cysteineeROS reactive oxygen speciesSAM S-adenosylmethioninefgMet methionine3 Biotech (2021) 11:hHcy homocy.
