Name :
Anti-B7-H3/CD276 antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
Rabbit polyclonal to B7-H3/CD276
Tested applications :
ELISA, WB, IP
Species reactivity :
Mouse B7-H3 / CD276
Alternative names :
B7-H3 antibody; B7H3 antibody; B7RP-2 antibody; Cd276 antibody; CD276 antibody; PSEC0249 antibody; 4Ig-B7-H3 antibody; 6030411F23Rik antibody; AU016588 antibody; B7h3 antibody; B7-H3 antibody
Immunogen :
Mouse B7-H3/CD276 (His Tag) recombinant protein
Isotype :
Rabbit IgG
Preparation :
Produced in rabbits immunized with purified, recombinant Mouse B7-H3 / CD276 (rh B7-H3 / CD276; NP_598744.1; Met1-Phe244). B7-H3 / CD276 specific IgG was purified by Mouse B7-H3 / CD276 affinity chromatography.
Clonality :
Polyclonal
Formulation :
0.2 μm filtered solution in PBS
Storage instructions :
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Applications :
WB: 2-10 μg/mlELISA:0.1-0.2 μg/mLThis antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse B7-H3 / CD276. The detection limit for Mouse B7-H3 / CD276 is IP: 1-4 μg/mg of lysate
Background :
B7-H3 is a member of the B7 family of immune regulatory ligands that is thought to attenuate peripheral immune responses through co-inhibition. It plays an important role in adaptive immune responses, and was shown to either promote or inhibit T-cell responses in various experimental systems. B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is a novel protein structurally related to the B7 family of ligands by the presence of a single set of immunoglobulin-V-like and immunoglobulin-C-like (VC) domains. Previous studies have correlated its overexpression with poor prognosis and decreased tumor-infiltrating lymphocytes in various carcinomas including uterine endometrioid carcinomas, and mounting evidence supports an immuno-inhibitory role in ovarian cancer prognosis. Recently, B7-H3 expression has been reported in several human cancers indicating an additional function of B7-H3 as a regulator of antitumor immunity.
References :
Suh WK, et al. (2004) The immune regulatory protein B7-H3 promotes osteoblast differentiation and bone mineralization. Proc Natl Acad Sci U S A. 101(35): 12969-73. Waschbisch A, et al. (2008) Human muscle cells express the costimulatory molecule B7-H3, which modulates muscle-immune interactions. Arthritis Rheum. 58(11): 3600-8. Loos M, et al. (2010) B7-h3 and its role in antitumor immunity. Clin Dev Immunol. 2010: 683875. Zang X, et al. (2010) Tumor associated endothelial expression of B7-H3 predicts survival in ovarian carcinomas. Mod Pathol. 23(8): 1104-12. Sun J, et al. (2010) Clinical significance and regulation of the costimulatory molecule B7-H3 in human colorectal carcinoma. Cancer Immunol Immunother. 59(8): 1163-71.
Related websites: https://www.medchemexpress.com/antibodies.html
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