Name :
GNPAT antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
GNPAT Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells. Positive IP detected in COLO 320 cells. Positive WB detected in HepG2 cells, COLO 320 cells, HeLa cells, human placenta tissue, mouse brain tissue, PC-3 cells, Transfected HEK-293 cells. Observed molecular weight by Western-blot: 65-69 kDa
Tested applications :
ELISA, IF, WB, IP
Species reactivity :
Human,Mouse,Rat; other species not tested.
Alternative names :
DAP AT antibody; DAPAT antibody; DHAP AT antibody; DHAPAT antibody; GNPAT antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of GNPAT recombinant protein (Accession Number: NM_014236). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:200-1:2000IP: 1:200-1:2000IF: 1:20-1:200
Background :
GNPAT, also named as DAPAT and DHAPAT, belongs to the GPAT/DAPAT family. It is a key enzyme in the biosynthesis of ether phospholipids. GNPAT is localized exclusively within peroxisomes. Full GNPAT activity depends not only on the presence of AGPS, but also on the integrity of substrate channeling from GNPAT to AGPS.(PMID:21990100) This antibody recognize the 69kd GNPAT protein.
References :
Itzkovitz B, Jiralerspong S, Nimmo G. Functional characterization of novel mutations in GNPAT and AGPS, causing rhizomelic chondrodysplasia punctata (RCDP) types 2 and 3. Human mutation. 33(1):189-97. 2012. Karnati S, Palaniswamy S, Alam MR, Oruqaj G, Stamme C, Baumgart-Vogt E. C22-bronchial and T7-alveolar epithelial cell lines of the immortomouse are excellent murine cell culture model systems to study pulmonary peroxisome biology and metabolism. Histochemistry and cell biology. 145(3):287-304. 2016.
Related websites: https://www.medchemexpress.com/antibodies.html
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