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Name :
GORASP2 antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
GORASP2 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human colon cancer tissue. Positive WB detected in HeLa cells, rat testis tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 59 kDa

Tested applications :

Species reactivity :
Human,Mouse,Rat; other species not tested.

Alternative names :
DKFZp434D156 antibody; FLJ13139 antibody; Golgi phosphoprotein 6 antibody; GOLPH6 antibody; GORASP2 antibody; GRASP55 antibody; GRS2 antibody; p59 antibody

Immunogen :

Isotype :
Rabbit IgG

Preparation :
This antibody was obtained by immunization of GORASP2 recombinant protein (Accession Number: NM_015530). Purification method: Antigen affinity purified.

Clonality :

Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:200-1:2000IP: 1:200-1:2000IHC: 1:20-1:200IF: 1:20-1:200

Background :
GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.

References :
Dupont N, Jiang S, Pilli M, Ornatowski W, Bhattacharya D, Deretic V. Autophagy-based unconventional secretory pathway for extracellular delivery of IL-1β. The EMBO journal. 30(23):4701-11. 2011. Xiang Y, Zhang X, Nix DB. Regulation of protein glycosylation and sorting by the Golgi matrix proteins GRASP55/65. Nature communications. 4:1659. 2013. Zhang X, Zhang H, Wang Y. Phosphorylation regulates VCIP135 function in Golgi membrane fusion during the cell cycle. Journal of cell science. 127(Pt 1):172-81. 2014. Tian G, Ropelewski P, Nemet I, Lee R, Lodowski KH, Imanishi Y. An unconventional secretory pathway mediates the cilia targeting of peripherin/rds. The Journal of neuroscience : the official journal of the Society for Neuroscience. 34(3):992-1006. 2014. Lee I, Tiwari N, Dunlop MH, Graham M, Liu X, Rothman JE. Membrane adhesion dictates Golgi stacking and cisternal morphology. Proceedings of the National Academy of Sciences of the United States of America. 111(5):1849-54. 2014. Ferraro F, Kriston-Vizi J, Metcalf DJ. A two-tier Golgi-based control of organelle size underpins the functional plasticity of endothelial cells. Developmental cell. 29(3):292-304. 2014. Lavieu G, Dunlop MH, Lerich A, Zheng H, Bottanelli F, Rothman JE. The Golgi ribbon structure facilitates anterograde transport of large cargoes. Molecular biology of the cell. 25(19):3028-36. 2014. Xiang Y, Wang Y. GRASP55 and GRASP65 play complementary and essential roles in Golgi cisternal stacking. The Journal of cell biology. 188(2):237-51. 2010.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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Author: Gardos- Channel