Name :
GPR161 antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
GPR161 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells. Positive IHC detected in human placenta tissue. Observed molecular weight by Western-blot: 70 kDa
Tested applications :
ELISA, WB, IHC
Species reactivity :
Human,Mouse,Rat; other species not tested.
Alternative names :
FLJ33952 antibody; G protein coupled receptor 161 antibody; G protein coupled receptor RE2 antibody; GPR161 antibody; RE2 antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of GPR161 recombinant protein (Accession Number: NM_001375883). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:500-1:5000IHC: 1:20-1:200
Background :
GPR161 (also known as RE2) is an orphan G protein-coupled receptor and plays important roles in the Hh pathway. In the absence of Hh signals, GPR161 localizes to primary cilia and keeps the downstream GLI transcription factors in their repressor forms (PMID: 26305592). Ciliary localization of GPR161 requires TULP3 and the IFT-A complex. In presence of SHH, GPR161 is removed from primary cilia and is internalized into recycling endosomes, preventing its activity and allowing activation of the Shh signaling.
References :
Garcia-Gonzalo FR, Phua SC, Roberson EC. Phosphoinositides Regulate Ciliary Protein Trafficking to Modulate Hedgehog Signaling. Developmental cell. 34(4):400-9. 2015. Yee LE, Garcia-Gonzalo FR, Bowie RV. Conserved Genetic Interactions between Ciliopathy Complexes Cooperatively Support Ciliogenesis and Ciliary Signaling. PLoS genetics. 11(11):e1005627. 2015.
Related websites: https://www.medchemexpress.com/antibodies.html
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