Name :
GST tag antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
GST tag Mouse Monoclonal antibody. Positive WB detected in recombinant protein. Positive IP detected in Recombinant protein protein.
Tested applications :
ELISA, WB, IP
Species reactivity :
Recombinant protein, Schistosoma japonicum; other species not tested.
Alternative names :
Glutathione S-Transferase antibody; gst antibody; GST tag antibody
Immunogen :
Isotype :
Mouse IgG2a
Preparation :
This antibody was obtained by immunization of Recombinant Protein (Accession Number: FN315687). Purification method: Protein A purified.
Clonality :
Monoclonal
Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:5000-1:50000IP: 1:5000-1:50000
Background :
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. This antibody recognizes the GST-tag fused to either the amino- or carboxy-terminus of targeted proteins.
References :
Wu K, Xue X, Li M. High level expression, purification and characterization of recombinant CCR5 as a vaccine candidate against HIV. Protein expression and purification. 89(2):124-30. 2013. Xu XH, Deng CY, Liu Y. MARCKS regulates membrane targeting of Rab10 vesicles to promote axon development. Cell research. 24(5):576-94. 2014. Li C, Wang L, Zhang J. CERKL interacts with mitochondrial TRX2 and protects retinal cells from oxidative stress-induced apoptosis. Biochimica et biophysica acta. 1842(7):1121-9. 2014. Hao J, Shen R, Li Y, Zhang Y, Yin Y. Cancer-testis antigen HCA587/MAGE-C2 interacts with BS69 and promotes its degradation in the ubiquitin-proteasome pathway. Biochemical and biophysical research communications. 449(4):386-91. 2014. Guan JJ, Zhang XD, Sun W, Qi L, Wu JC, Qin ZH. DRAM1 regulates apoptosis through increasing protein levels and lysosomal localization of BAX. Cell death & disease. 6:e1624. 2015. Ji S, Qin Y, Shi S. ERK kinase phosphorylates and destabilizes the tumor suppressor FBW7 in pancreatic cancer. Cell research. 25(5):561-73. 2015. Qin S, Zhang B, Tian W, Gu L, Lu Z, Deng D. Kaiso mainly locates in the nucleus in vivo and binds to methylated, but not hydroxymethylated DNA. Chinese journal of cancer research = Chung-kuo yen cheng yen chiu. 27(2):148-55. 2015. Liu C, Zhang D, Shen Y. DPF2 regulates OCT4 protein level and nuclear distribution. Biochimica et biophysica acta. 1853(12):3279-93. 2015.
Related websites: https://www.medchemexpress.com/antibodies.html
Popular product recommendations:
Phospho-CDC37 (Ser13) Antibody (YA219)
Phospho-mTOR (Ser2481) Antibody
StAR Antibody: StAR Antibody is an unconjugated, approximately 32 kDa, rabbit-derived, anti-StAR polyclonal antibody. StAR Antibody can be used for: WB, IHC-P, IHC-F, Flow-Cyt, IF expriments in human, mouse, rat, and predicted: dog, cow, horse background without labeling.
