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Name :
GZMB antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
GZMB Rabbit Polyclonal antibody. Positive WB detected in HL-60 cells. Positive IHC detected in human lymphoma tissue. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 33kd

Tested applications :

Species reactivity :
Human; other species not tested.

Alternative names :
CCPI antibody; CGL 1 antibody; CGL1 antibody; CSP B antibody; CSPB antibody; CTLA1 antibody; CTSGL1 antibody; Endogenous granzyme B antibody; Granzyme B antibody; GZMB antibody; HLP antibody; SECT antibody

Immunogen :

Isotype :
Rabbit IgG

Preparation :
This antibody was obtained by immunization of GZMB recombinant protein (Accession Number: NM_004131). Purification method: Antigen affinity purified.

Clonality :

Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:500-1:5000IHC: 1:20-1:200IF: 1:20-1:200

Background :
GZMB(Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization(PMID:17283187).GzmB induces laminB degradation in isolated nuclei less efficiently than GzmA(PMID:11331782).This full length protein has 2 glycosylation sites and a signal peptide.Unglycosylated human granzyme B is 26 kDa and g high mannose glycosylated is 32 kDa and only 32kDa or smaller forms of granzyme B are accumulated within nuclei(PMID:8626751).

References :
Li P, Zheng G, Yang Y. Granzyme B is recovered by natural killer cells via clathrin-dependent endocytosis. Cellular and molecular life sciences : CMLS. 67(18):3197-208. 2010. Cho YT, Lin JW, Chen YC. Generalized bullous fixed drug eruption is distinct from Stevens-Johnson syndrome/toxic epidermal necrolysis by immunohistopathological features. Journal of the American Academy of Dermatology. 70(3):539-48. 2014.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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Author: Gardos- Channel