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Name :
HDAC5-specific antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
HDAC5-specific Rabbit Polyclonal antibody. Positive IHC detected in human brain tissue, human heart tissue, mouse brain tissue. Positive WB detected in human brain tissue, HEK-293 cells, HeLa cells, MCF-7 cells, rat brain tissue. Observed molecular weight by Western-blot: 124kd

Tested applications :

Species reactivity :
Human, Mouse, Rat; other species not tested.

Alternative names :
Antigen NY CO 9 antibody; FLJ90614 antibody; HD5 antibody; HDAC5 antibody; histone deacetylase 5 antibody; KIAA0600 antibody; NY CO 9 antibody

Immunogen :

Isotype :
Rabbit IgG

Preparation :
This antibody was obtained by immunization of Peptide (Accession Number: XM_005256906). Purification method: Antigen affinity purified.

Clonality :

Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:200-1:1000IHC: 1:20-1:200

Background :
Histone acetylation and deacetylation alternately exposes and occludes DNA to transcription factors. At least 4 classes of HDAC were identified. HDAC5 is a class II HDAC. HDAC5 responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC5 is involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, HDAC5 shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. This antibody only binds HDAC5. It does not cross-react with other HDACs.

References :
He M, Zhang B, Wei X. HDAC4/5-HMGB1 signalling mediated by NADPH oxidase activity contributes to cerebral ischaemia/reperfusion injury. Journal of cellular and molecular medicine. 17(4):531-42. 2013. Wang X, Liu J, Zhen J. Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy. Kidney international. 86(4):712-25. 2014. Fang J, Li Y, Zhou K, Hua Y, Wang C, Mu D. Antithetical regulation of α-myosin heavy chain between fetal and adult heart failure though shuttling of HDAC5 regulating YY-1 function. Cardiovascular toxicology. 15(2):147-56. 2015.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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Author: Gardos- Channel