Name :
HDAC8 antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
HDAC8 Rabbit Polyclonal antibody. Positive IP detected in Raji cells. Positive WB detected in HeLa cells, Raji cells. Observed molecular weight by Western-blot: 49kd
Tested applications :
ELISA, WB, IP
Species reactivity :
Human,Mouse,Rat; other species not tested.
Alternative names :
HD8 antibody; HDAC8 antibody; HDACL1 antibody; histone deacetylase 8 antibody; RPD3 antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of HDAC8 recombinant protein (Accession Number: NM_018486). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:500-1:5000IP: 1:200-1:2000
Background :
Histone deacetylases (HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 8 was primarily found in the nucleus. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. This antibody is a rabbit polyclonal antibody raised against full length HDAC8 of human origin.
References :
Shu L, Khor TO, Lee JH. Epigenetic CpG demethylation of the promoter and reactivation of the expression of Neurog1 by curcumin in prostate LNCaP cells. The AAPS journal. 13(4):606-14. 2011. Wu TY, Khor TO, Su ZY. Epigenetic modifications of Nrf2 by 3,3′-diindolylmethane in vitro in TRAMP C1 cell line and in vivo TRAMP prostate tumors. The AAPS journal. 15(3):864-74. 2013. Shen Q, Yao Q, Sun J. Downregulation of histone deacetylase 1 by microRNA-520h contributes to the chemotherapeutic effect of doxorubicin. FEBS letters. 588(1):184-91. 2014. Paredes-Gonzalez X, Fuentes F, Su ZY, Kong AN. Apigenin reactivates Nrf2 anti-oxidative stress signaling in mouse skin epidermal JB6 P + cells through epigenetics modifications. The AAPS journal. 16(4):727-35. 2014. Wang X, Liu J, Zhen J. Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy. Kidney international. 86(4):712-25. 2014. Guo Y, Shu L, Zhang C, Su ZY, Kong AN. Curcumin inhibits anchorage-independent growth of HT29 human colon cancer cells by targeting epigenetic restoration of the tumor suppressor gene DLEC1. Biochemical pharmacology. 94(2):69-78. 2015.
Related websites: https://www.medchemexpress.com/antibodies.html
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