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Name :
Histone H1.0 antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
Histone H1.0 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IP detected in A431 cells. Positive WB detected in A431 cells, HeLa cells, Jurkat cells, mouse spleen tissue, rat spleen tissue. Observed molecular weight by Western-blot: 32kd

Tested applications :

Species reactivity :
Human,Mouse,Rat; other species not tested.

Alternative names :
H1 histone family antibody; member 0 antibody; H10 antibody; H1F0 antibody; H1FV antibody; Histone H1 antibody; Histone H1(0) antibody; Histone H1.0 antibody

Immunogen :

Isotype :
Rabbit IgG

Preparation :
This antibody was obtained by immunization of Histone H1.0 recombinant protein (Accession Number: NM_005318). Purification method: Antigen affinity purified.

Clonality :

Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:500-1:5000IP: 1:200-1:2000IF: 1:20-1:200

Background :
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.Linker histones are involved in the formation of higher order structure in chromatin and the maintenance of overall chromatin compaction. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.Histone H1.0 (H1F0,H1FV) is a linker histone that is widely expressed in many tissues and almost all vertebrates, unlike some other linker histones. The observed molecular weight of H1F0 is about 32 kDa.

References :
Poolman TM, Farrow SN, Matthews L, Loudon AS, Ray DW. Pin1 promotes GR transactivation by enhancing recruitment to target genes. Nucleic acids research. 41(18):8515-25. 2013. Tao M, Liu L, Shen M. Inflammatory stimuli promote growth and invasion of pancreatic cancer cells through NF-κB pathway dependent repression of PP2Ac. Cell cycle (Georgetown, Tex.). 15(3):381-93. 2016.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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Author: Gardos- Channel