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Name :
Anti-beta Actin Rabbit antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
beta Actin Rabbit polyclonal antibody

Tested applications :

Species reactivity :
Human, Mouse, Rat

Alternative names :

Immunogen :

Isotype :
Rabbit IgG

Preparation :
Antigen: A synthesized peptide derived from human beta Actin

Clonality :

Formulation :
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage instructions :
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Applications :
WB: 1/3000-1/10000IHC: 1/50-1/200ICC/IF: 1/50-1/200

Background :
Swiss-Prot Acc.P60709.beta-Actin is one of six different actin isoforms that have been identified. The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties. Therefore, Antibodies against beta-Actin are useful as loading controls for Western Blotting. However it should be noted that levels of beta-Actin may not be stable in certain cells. For example, expression of beta-Actin in adipose tissue is very low and therefore it should not be used as loading control for these tissues.

References :

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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Author: Gardos- Channel