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Name :
HLA-F antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
HLA-F Rabbit Polyclonal antibody. Positive IHC detected in human skin cancer tissue, human placenta tissue, human spleen tissue. Positive WB detected in human placenta tissue, A431 cells, human spleen tissue, Raji cells. Observed molecular weight by Western-blot: 45 kDa

Tested applications :

Species reactivity :
Human; other species not tested.

Alternative names :
CDA12 antibody; HLA 5.4 antibody; HLA CDA12 antibody; HLA F antibody; HLA F antigen antibody; HLAF antibody; HLA-F antibody; LeukOCyte antigen F antibody; MHC class I antigen F antibody

Immunogen :

Isotype :
Rabbit IgG

Preparation :
This antibody was obtained by immunization of HLA-F recombinant protein (Accession Number: NM_018950). Purification method: Antigen affinity purified.

Clonality :

Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:200-1:2000IHC: 1:20-1:200

Background :
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-F is a non-classical MHC class I molecule. (PMID: 667938; 3375250; 2249951)

References :
Ishigami S, Arigami T, Setoyama T. Clinical-pathological implication of human leukocyte antigen-F-positive gastric adenocarcinoma. The Journal of surgical research. 184(2):802-6. 2013. Zhang X, Lin A, Zhang JG. Alteration of HLA-F and HLA I antigen expression in the tumor is associated with survival in patients with esophageal squamous cell carcinoma. International journal of cancer. 132(1):82-9. 2013. Zhang JG, Zhang X, Lin A, Yan WH. Lesion HLA-F expression is irrelevant to prognosis for patients with gastric cancer. Human immunology. 74(7):828-32. 2013. Lin A, Zhang X, Ruan YY, Wang Q, Zhou WJ, Yan WH. HLA-F expression is a prognostic factor in patients with non-small-cell lung cancer. Lung cancer (Amsterdam, Netherlands). 74(3):504-9. 2011.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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Author: Gardos- Channel