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Name :
HNRNPA1 antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
HNRNPA1 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, HeLa cells, mouse colon tissue. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 34kd

Tested applications :

Species reactivity :
Human, Mouse, Pig; other species not tested.

Alternative names :
Helix destabilizing protein antibody; hnRNP A1 antibody; hnRNP core protein A1 antibody; HNRNPA1 antibody; HNRPA1 antibody

Immunogen :
Human HNRNPA1 Recombinant protein (GST tag)

Isotype :
Rabbit IgG

Preparation :
This antibody was obtained by immunization of HNRNPA1 recombinant protein (Accession Number: NM_002136). Purification method: Antigen affinity purified.

Clonality :

Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:200-1:1000IHC: 1:20-1:200

Background :
In eukaryotic cells, nascent RNA polymerase II transcripts are associated in the nucleus with specific proteins to form ribonucleoprotein complexes called HNRP or 40S. Protein moiety of the HNRP particle has 6 major components called core proteins, and HNRPA1 is one of them. hnRNP A1 is a multifunctional RNA binding protein which is involved in pre-mRNA splicing, export of mature transcripts from the nucleus, mRNA turnover, and internal ribosome entry site (IRES)-mediated translation. HNRNPA1 has three isoforms with MW 30 kDa, 34 kDa and 39 kDa.

References :
Guo R, Li Y, Ning J, Sun D, Lin L, Liu X. HnRNP A1/A2 and SF2/ASF regulate alternative splicing of interferon regulatory factor-3 and affect immunomodulatory functions in human non-small cell lung cancer cells. PloS one. 8(4):e62729. 2013. Chen K, Huang C, Yuan J, Cheng H, Zhou R. Long-term artificial selection reveals a role of TCTP in autophagy in mammalian cells. Molecular biology and evolution. 31(8):2194-211. 2014. Markus MA, Marques FZ, Morris BJ. Resveratrol, by modulating RNA processing factor levels, can influence the alternative splicing of pre-mRNAs. PloS one. 6(12):e28926. 2011. Lu Q, Bai J, Zhang L. Two-dimensional liquid chromatography-tandem mass spectrometry coupled with isobaric tags for relative and absolute quantification (iTRAQ) labeling approach revealed first proteome profiles of pulmonary alveolar macrophages infected with porcine reproductive and respiratory syndrome virus. Journal of proteome research. 11(5):2890-903. 2012.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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Author: Gardos- Channel