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Name :
HOOK1 antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
HOOK1 Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, human brain tissue. Positive IP detected in mouse brain tissue. Positive IF detected in HEK-293 cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 85 kDa

Tested applications :

Species reactivity :
Human, Mouse; other species not tested.

Alternative names :
h hook1 antibody; hHK1 antibody; HK1 antibody; hook homolog 1 (Drosophila) antibody; HOOK1 antibody; Protein Hook homolog 1 antibody

Immunogen :

Isotype :
Rabbit IgG

Preparation :
This antibody was obtained by immunization of HOOK1 recombinant protein (Accession Number: NM_015888). Purification method: Antigen affinity purified.

Clonality :

Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:200-1:2000IP: 1:200-1:2000IHC: 1:20-1:200IF: 1:10-1:100

Background :
HOOK1, an orthologue of the Drosophila Hook protein, is a member of the microtubule-binding HOOK family of cytosolic coiled-coil proteins (HOOK1, HOOK2, HOOK3). These proteins contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. HOOK1 is required for spermatid differentiation. It is probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton. HOOK1 is a component of the FTS/Hook/FHIP complex which functions to promote vesicle trafficking and/or fusion via the HOPS (homotypic vesicular protein sorting) complex.

References :
Zhou J, Du YR, Qin WH. RIM-BP3 is a manchette-associated protein essential for spermiogenesis. Development (Cambridge, England). 136(3):373-82. 2009. Hall EA, Keighren M, Ford MJ. Acute versus chronic loss of mammalian Azi1/Cep131 results in distinct ciliary phenotypes. PLoS genetics. 9(12):e1003928. 2013.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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Author: Gardos- Channel