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Name :
HRAS-Specific antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
HRAS-Specific Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, NIH/3T3 cells, PC-3 cells. Positive FC detected in HeLa cells. Positive IHC detected in human kidney tissue, human prostate hyperplasia tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 21 kDa

Tested applications :

Species reactivity :
Human, Mouse; other species not tested.

Alternative names :
C BAS/HAS antibody; C H RAS antibody; C HA RAS1 antibody; CTLO antibody; GTPase HRas antibody; H Ras 1 antibody; H RASIDX antibody; Ha Ras antibody; HAMSV antibody; HRAS antibody; HRAS1 antibody; K RAS antibody; p21ras antibody; RASH1 antibody; Transforming protein p21 antibody

Immunogen :

Isotype :
Rabbit IgG

Preparation :
This antibody was obtained by immunization of Peptide (Accession Number: NM_176795). Purification method: Antigen affinity purified.

Clonality :

Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:200-1:1000IHC: 1:20-1:200IF: 1:10-1:100

Background :
HRAS,also named as c-bas/has and RASH1, is v-Ha-ras Harvey rat sarcoma viral oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. Defects in HRAS are a cause of cancer. The antibody is specific to HRAS, can’t recognize NRAS and KRAS.

References :
Cai WY, Wei TZ, Luo QC. The Wnt-β-catenin pathway represses let-7 microRNA expression through transactivation of Lin28 to augment breast cancer stem cell expansion. Journal of cell science. 126(Pt 13):2877-89. 2013. Ceccarelli V, Nocentini G, Billi M. Eicosapentaenoic acid activates RAS/ERK/C/EBPβ pathway through H-Ras intron 1 CpG island demethylation in U937 leukemia cells. PloS one. 9(1):e85025. 2014. Liu W, Ning R, Chen RN. Aspafilioside B induces G2/M cell cycle arrest and apoptosis by up-regulating H-Ras and N-Ras via ERK and p38 MAPK signaling pathways in human hepatoma HepG2 cells. Molecular carcinogenesis. 2015. Park SJ, Shim JW, Park HS. MacroH2A1 downregulation enhances the stem-like properties of bladder cancer cells by transactivation of Lin28B. Oncogene. 2015.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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Author: Gardos- Channel