Name :
HRAS-Specific antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
HRAS-Specific Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, NIH/3T3 cells, PC-3 cells. Positive FC detected in HeLa cells. Positive IHC detected in human kidney tissue, human prostate hyperplasia tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 21 kDa
Tested applications :
ELISA, IF, IHC, WB, FC
Species reactivity :
Human, Mouse; other species not tested.
Alternative names :
C BAS/HAS antibody; C H RAS antibody; C HA RAS1 antibody; CTLO antibody; GTPase HRas antibody; H Ras 1 antibody; H RASIDX antibody; Ha Ras antibody; HAMSV antibody; HRAS antibody; HRAS1 antibody; K RAS antibody; p21ras antibody; RASH1 antibody; Transforming protein p21 antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of Peptide (Accession Number: NM_176795). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:200-1:1000IHC: 1:20-1:200IF: 1:10-1:100
Background :
HRAS,also named as c-bas/has and RASH1, is v-Ha-ras Harvey rat sarcoma viral oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. Defects in HRAS are a cause of cancer. The antibody is specific to HRAS, can’t recognize NRAS and KRAS.
References :
Cai WY, Wei TZ, Luo QC. The Wnt-β-catenin pathway represses let-7 microRNA expression through transactivation of Lin28 to augment breast cancer stem cell expansion. Journal of cell science. 126(Pt 13):2877-89. 2013. Ceccarelli V, Nocentini G, Billi M. Eicosapentaenoic acid activates RAS/ERK/C/EBPβ pathway through H-Ras intron 1 CpG island demethylation in U937 leukemia cells. PloS one. 9(1):e85025. 2014. Liu W, Ning R, Chen RN. Aspafilioside B induces G2/M cell cycle arrest and apoptosis by up-regulating H-Ras and N-Ras via ERK and p38 MAPK signaling pathways in human hepatoma HepG2 cells. Molecular carcinogenesis. 2015. Park SJ, Shim JW, Park HS. MacroH2A1 downregulation enhances the stem-like properties of bladder cancer cells by transactivation of Lin28B. Oncogene. 2015.
Related websites: https://www.medchemexpress.com/antibodies.html
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