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Name :
HRP-conjugated alpha Tubulin antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
HRP-conjugated alpha Tubulin Mouse Monoclonal antibody. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 52kd

Tested applications :

Species reactivity :
Human,Mouse,Rat; other species not tested.

Alternative names :
Alpha tubulin ubiquitous antibody; alpha tubulin antibody; a tubulin antibody; HRP Tubulin Alpha antibody; K ALPHA 1 antibody; TUBA1B antibody; tubulin antibody; Tubulin Alpha antibody; Tubulin alpha 1B chain antibody; Tubulin alpha ubiquitous chain antibody; Tubulin K alpha 1 antibody; tubulin antibody; alpha 1b antibody

Immunogen :

Isotype :
Mouse IgG2b

Preparation :
This antibody was obtained by immunization of Recombinant Protein (Accession Number: NM_006082). Purification method: Protein A Purified.

Clonality :

Formulation :
PBS with 0.02% Thimerosal, 50% Glycerol, pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:2000-1:10000

Background :
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs. This antiody recognize Tubulin-Alpha only and it is conjugated with HRP. Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.

References :

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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Author: Gardos- Channel