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Name :
HRP-conjugated beta Actin antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
HRP-conjugated beta Actin Mouse Monoclonal antibody. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 42 kDa

Tested applications :

Species reactivity :
Human, Mouse, Rat, Zebra Fish; other species not tested.

Alternative names :
ACTB antibody; Actin antibody; actin antibody; beta antibody; Actin antibody; cytoplasmic 1 antibody; B actin antibody; Beta actin antibody; F actin antibody; HRP β actin antibody; HRP-ACTB antibody; HRP-Beta actin antibody; HRP-F actin antibody; PS1TP5BP1 antibody; β actin antibody

Immunogen :

Isotype :
Mouse IgM

Preparation :
This antibody was obtained by immunization of Recombinant Protein (Accession Number: NM_001101). Purification method: Caprylic acid/ammonium sulfate precipitation.

Clonality :

Formulation :
PBS with 0.02% Thimerosal, 50% Glycerol, pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:2000-1:20000

Background :
Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody can recognize all of actins. This antibody is conjugated with HRP. Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.

References :

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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Author: Gardos- Channel