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Name :
Hsc70 antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
Hsc70 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, A431 cells, C6 cells, COLO 320 cells, HeLa cells, HepG2 cells, MCF7 cells, NIH/3T3 cells, rat brain tissue. Positive IHC detected in human ovary tumor tissue, human gliomas tissue. Observed molecular weight by Western-blot: 70-73 kDa

Tested applications :

Species reactivity :
Human,Mouse,Rat; other species not tested.

Alternative names :
HSC54 antibody; HSC70 antibody; HSC71 antibody; HSP71 antibody; HSP73 antibody; HSPA10 antibody; HSPA8 antibody

Immunogen :

Isotype :
Rabbit IgG

Preparation :
This antibody was obtained by immunization of Hsc70 recombinant protein (Accession Number: BC007276). Purification method: Antigen affinity purified.

Clonality :

Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:1000-1:10000IP: 1:200-1:2000IHC: 1:20-1:200

Background :
HSPA8 (also known as HSC70) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA8 is a constitutively expressed cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Recently it has been reported that HSPA8 can interact with α-synuclein, the critical pathological protein of Parkinson’s disease, indicating its implication in neurodegenerative disease. (21832061)

References :
Han B, Zhou H, Jia G. MAPKs and Hsc70 are critical to the protective effect of molecular hydrogen during the early phase of acute pancreatitis. The FEBS journal. 283(4):738-56. 2016. Su CC, Su JH, Lin JJ. An investigation into the cytotoxic effects of 13-acetoxysarcocrassolide from the soft coral Sarcophyton crassocaule on bladder cancer cells. Marine drugs. 9(12):2622-42. 2011. Liu CI, Wang RY, Lin JJ. Proteomic profiling of the 11-dehydrosinulariolide-treated oral carcinoma cells Ca9-22: effects on the cell apoptosis through mitochondrial-related and ER stress pathway. Journal of proteomics. 75(18):5578-89. 2012. Wang BS, Yang Y, Yang H. PKCι counteracts oxidative stress by regulating Hsc70 in an esophageal cancer cell line. Cell stress & chaperones. 18(3):359-66. 2013. Vakifahmetoglu-Norberg H, Kim M, Xia HG. Chaperone-mediated autophagy degrades mutant p53. Genes & development. 27(15):1718-30. 2013. Ma J, Hu Y, Guo M, Huang Z, Li W, Wu Y. hERG potassium channel blockage by scorpion toxin BmKKx2 enhances erythroid differentiation of human leukemia cells K562. PloS one. 8(12):e84903. 2013. Liu YZ, Jiang YY, Wang BS. A panel of protein markers for the early detection of lung cancer with bronchial brushing specimens. Cancer cytopathology. 122(11):833-41. 2014. Liu C, Lin JJ, Yang ZY, Tsai CC, Hsu JL, Wu YJ. Proteomic study reveals a co-occurrence of gallic acid-induced apoptosis and glycolysis in B16F10 melanoma cells. Journal of agricultural and food chemistry. 62(48):11672-80. 2014.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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Author: Gardos- Channel