Name :
Hsc70 antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
Hsc70 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, A431 cells, C6 cells, COLO 320 cells, HeLa cells, HepG2 cells, MCF7 cells, NIH/3T3 cells, rat brain tissue. Positive IHC detected in human ovary tumor tissue, human gliomas tissue. Observed molecular weight by Western-blot: 70-73 kDa
Tested applications :
ELISA, IHC, IP, WB
Species reactivity :
Human,Mouse,Rat; other species not tested.
Alternative names :
HSC54 antibody; HSC70 antibody; HSC71 antibody; HSP71 antibody; HSP73 antibody; HSPA10 antibody; HSPA8 antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of Hsc70 recombinant protein (Accession Number: BC007276). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:1000-1:10000IP: 1:200-1:2000IHC: 1:20-1:200
Background :
HSPA8 (also known as HSC70) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA8 is a constitutively expressed cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Recently it has been reported that HSPA8 can interact with α-synuclein, the critical pathological protein of Parkinson’s disease, indicating its implication in neurodegenerative disease. (21832061)
References :
Han B, Zhou H, Jia G. MAPKs and Hsc70 are critical to the protective effect of molecular hydrogen during the early phase of acute pancreatitis. The FEBS journal. 283(4):738-56. 2016. Su CC, Su JH, Lin JJ. An investigation into the cytotoxic effects of 13-acetoxysarcocrassolide from the soft coral Sarcophyton crassocaule on bladder cancer cells. Marine drugs. 9(12):2622-42. 2011. Liu CI, Wang RY, Lin JJ. Proteomic profiling of the 11-dehydrosinulariolide-treated oral carcinoma cells Ca9-22: effects on the cell apoptosis through mitochondrial-related and ER stress pathway. Journal of proteomics. 75(18):5578-89. 2012. Wang BS, Yang Y, Yang H. PKCι counteracts oxidative stress by regulating Hsc70 in an esophageal cancer cell line. Cell stress & chaperones. 18(3):359-66. 2013. Vakifahmetoglu-Norberg H, Kim M, Xia HG. Chaperone-mediated autophagy degrades mutant p53. Genes & development. 27(15):1718-30. 2013. Ma J, Hu Y, Guo M, Huang Z, Li W, Wu Y. hERG potassium channel blockage by scorpion toxin BmKKx2 enhances erythroid differentiation of human leukemia cells K562. PloS one. 8(12):e84903. 2013. Liu YZ, Jiang YY, Wang BS. A panel of protein markers for the early detection of lung cancer with bronchial brushing specimens. Cancer cytopathology. 122(11):833-41. 2014. Liu C, Lin JJ, Yang ZY, Tsai CC, Hsu JL, Wu YJ. Proteomic study reveals a co-occurrence of gallic acid-induced apoptosis and glycolysis in B16F10 melanoma cells. Journal of agricultural and food chemistry. 62(48):11672-80. 2014.
Related websites: https://www.medchemexpress.com/antibodies.html
Popular product recommendations:
Trontinemab
Pagibaximab
BRD2 Antibody: BRD2 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 88 kDa, targeting to BRD2. It can be used for WB,ICC,IHC-P,FC assays with tag free, in the background of Human.
