Name :
LDHB antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
LDHB Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue, DU 145 cells, PC-3 cells, rat brain tissue, rat liver tissue. Positive FC detected in HeLa cells. Positive IHC detected in human prostate tissue. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 35 kDa
Tested applications :
ELISA, WB, IHC, IF, FC, IP
Species reactivity :
Human,Mouse,Rat; other species not tested.
Alternative names :
lactate dehydrogenase B antibody; LDH B antibody; LDH H antibody; LDH heart subunit antibody; LDHB antibody; TRG 5 antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of LDHB recombinant protein (Accession Number: NM_002300). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:1000-1:10000IP: 1:500-1:5000IHC: 1:20-1:200IF: 1:20-1:200
Background :
LDHB, also named as LDH-H and NY-REN-46, belongs to the LDH/MDH superfamily. And LDH family. It is an enzyme which catalyzes the reversible conversion of lactate and pyruvate, and NAD and NADH, in the glycolytic pathway.It is glycolytic enzymes and it is positively regulated by mTOR.
References :
Sun Q, Chen X, Ma J. Mammalian target of rapamycin up-regulation of pyruvate kinase isoenzyme type M2 is critical for aerobic glycolysis and tumor growth. Proceedings of the National Academy of Sciences of the United States of America. 108(10):4129-34. 2011. Izquierdo-Álvarez A, Ramos E, Villanueva J. Differential redox proteomics allows identification of proteins reversibly oxidized at cysteine residues in endothelial cells in response to acute hypoxia. Journal of proteomics. 75(17):5449-62. 2012. Chen AP, Chu W, Gu YP, Cunningham CH, Cunnhingham CH. Probing early tumor response to radiation therapy using hyperpolarized Chung SH, Shen W, Gillies MC. Laser capture microdissection-directed profiling of glycolytic and mTOR pathways in areas of selectively ablated Müller cells in the murine retina. Investigative ophthalmology & visual science. 54(10):6578-85. 2013.
Related websites: https://www.medchemexpress.com/antibodies.html
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