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Name :
METTL3 antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
METTL3 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, HeLa cells, HepG2 cells. Observed molecular weight by Western-blot: 70 kDa

Tested applications :
ELISA, WB, IP

Species reactivity :
Human,Mouse,Rat; other species not tested.

Alternative names :
IME4 antibody; M6A antibody; methyltransferase like 3 antibody; METTL3 antibody; MT A70 antibody; MTA70 antibody; Spo8 antibody

Immunogen :
Human METTL3 Recombinant protein (His tag)

Isotype :
Rabbit IgG

Preparation :
This antibody was obtained by immunization of METTL3 recombinant protein (Accession Number: NM_019852). Purification method: Antigen affinity purified.

Clonality :
Polyclonal

Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:500-1:5000IP: 1:500-1:5000

Background :
METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs.

References :
Fustin JM, Doi M, Yamaguchi Y. RNA-methylation-dependent RNA processing controls the speed of the circadian clock. Cell. 155(4):793-806. 2013. Wang X, Lu Z, Gomez A. N6-methyladenosine-dependent regulation of messenger RNA stability. Nature. 505(7481):117-20. 2014. Liu J, Yue Y, Han D. A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation. Nature chemical biology. 10(2):93-5. 2014. Zhao X, Yang Y, Sun BF. FTO-dependent demethylation of N6-methyladenosine regulates mRNA splicing and is required for adipogenesis. Cell research. 24(12):1403-19. 2014. Batista PJ, Molinie B, Wang J. m(6)A RNA modification controls cell fate transition in mammalian embryonic stem cells. Cell stem cell. 15(6):707-19. 2014. Chen T, Hao YJ, Zhang Y. m(6)A RNA methylation is regulated by microRNAs and promotes reprogramming to pluripotency. Cell stem cell. 16(3):289-301. 2015.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
Related websites: https://www.medchemexpress.com/antibodies.html
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Author: Gardos- Channel