Name :
METTL3 antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
METTL3 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, HeLa cells, HepG2 cells. Observed molecular weight by Western-blot: 70 kDa
Tested applications :
ELISA, WB, IP
Species reactivity :
Human,Mouse,Rat; other species not tested.
Alternative names :
IME4 antibody; M6A antibody; methyltransferase like 3 antibody; METTL3 antibody; MT A70 antibody; MTA70 antibody; Spo8 antibody
Immunogen :
Human METTL3 Recombinant protein (His tag)
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of METTL3 recombinant protein (Accession Number: NM_019852). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:500-1:5000IP: 1:500-1:5000
Background :
METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs.
References :
Fustin JM, Doi M, Yamaguchi Y. RNA-methylation-dependent RNA processing controls the speed of the circadian clock. Cell. 155(4):793-806. 2013. Wang X, Lu Z, Gomez A. N6-methyladenosine-dependent regulation of messenger RNA stability. Nature. 505(7481):117-20. 2014. Liu J, Yue Y, Han D. A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation. Nature chemical biology. 10(2):93-5. 2014. Zhao X, Yang Y, Sun BF. FTO-dependent demethylation of N6-methyladenosine regulates mRNA splicing and is required for adipogenesis. Cell research. 24(12):1403-19. 2014. Batista PJ, Molinie B, Wang J. m(6)A RNA modification controls cell fate transition in mammalian embryonic stem cells. Cell stem cell. 15(6):707-19. 2014. Chen T, Hao YJ, Zhang Y. m(6)A RNA methylation is regulated by microRNAs and promotes reprogramming to pluripotency. Cell stem cell. 16(3):289-301. 2015.
Related websites: https://www.medchemexpress.com/antibodies.html
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