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Name :
MITF antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
MITF Rabbit Polyclonal antibody. Positive IP detected in mouse heart tissue. Positive WB detected in Jurkat cells, HeLa cells, mouse heart tissue, rat skin tissue. Positive FC detected in hESC cells. Observed molecular weight by Western-blot: 60-65kd

Tested applications :
ELISA, WB, FC, IP

Species reactivity :
Human,Mouse,Rat; other species not tested.

Alternative names :
bHLHe32 antibody; MITF antibody; WS2A antibody

Immunogen :

Isotype :
Rabbit IgG

Preparation :
This antibody was obtained by immunization of MITF recombinant protein (Accession Number: BC012503). Purification method: Antigen affinity purified.

Clonality :
Polyclonal

Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:1000-1:10000IP: 1:500-1:5000

Background :
The retinal pigment epithelium (RPE) has a essential role in maintaining visual function and dedifferentiation of RPE contributes to the pathophysiology of several ocular diseases[PMID: 22523078]. Microphthalmia-associated transcription factor (MITF) is a key regulator of RPE differentiation that is also down-regulated in dedifferentiated hfRPE cells. MITF is a basic helix-loop-helix (hHLH)-leucine zipper protein that involves in the development of various cell types, including neural crest-derived melanocytes and optic cup-derived retinal pigment epithelial cells [PMID: 10578055].

References :
Lee CS, Park M, Han J. Liver X receptor activation inhibits melanogenesis through the acceleration of ERK-mediated MITF degradation. The Journal of investigative dermatology. 133(4):1063-71. 2013. Won YM, Seong ZK, Kim JL. Triterpene glycosides with stimulatory activity on melanogenesis from the aerial parts of Weigela subsessilis. Archives of pharmacal research. 38(8):1541-51. 2015. Park J, Chung H, Bang SH. (E)-4-(3,4-Dimethoxyphenyl)but-3-en-1-ol Enhances Melanogenesis through Increasing Upstream Stimulating Factor-1-Mediated Tyrosinase Expression. PloS one. 10(11):e0141988. 2015.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
Related websites: https://www.medchemexpress.com/antibodies.html
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Author: Gardos- Channel