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Name :
GSPT1 antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
GSPT1 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue. Positive WB detected in SGC-7901 cells, HeLa cells. Observed molecular weight by Western-blot: 80 kDa; 84 kDa

Tested applications :

Species reactivity :
Human,Mouse,Rat; other species not tested.

Alternative names :
551G9.2 antibody; eRF3a antibody; ETF3A antibody; FLJ38048 antibody; FLJ39067 antibody; G1 to S phase transition 1 antibody; GSPT1 antibody; GST1 antibody

Immunogen :

Isotype :
Rabbit IgG

Preparation :
This antibody was obtained by immunization of GSPT1 recombinant protein (Accession Number: NM_001130006). Purification method: Antigen affinity purified.

Clonality :

Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:200-1:1000IHC: 1:20-1:200

Background :
The eukaryotic Release Factor 3 (eRF3) is a GTPase that associates with eRF1 in a complex that mediates translation termination. Eukaryotic release factor 3 (eRF3) has many functions in eukaryotic cells, such as controlling the regulation of the cell cycle at the G1 to S phase transition, and regulating protein synthesis as a GTP dependent stimulator of eRF1 in translation termination. It was also reported to play a key role as an initiator of the mRNA degradation machinery in the recycling of ribosomes in successive cycles of translation,and probably also in transcription regulation. GSPT1 is one subunit of eRF3[PMID:15917414,12923185]. It involves in translation termination in response to the termination codons UAA, UAG and UGA and stimulates the activity of ERF1.

References :
Teng S, Gao L, Paajanen V, Pu J, Fan Z. Readthrough of nonsense mutation W822X in the SCN5A gene can effectively restore expression of cardiac Na+ channels. Cardiovascular research. 83(3):473-80. 2009. Nair S, Bora-Singhal N, Perumal D, Chellappan S. Nicotine-mediated invasion and migration of non-small cell lung carcinoma cells by modulating STMN3 and GSPT1 genes in an ID1-dependent manner. Molecular cancer. 13:173. 2014. Terui Y, Sakamoto A, Yoshida T. Polyamine stimulation of eEF1A synthesis based on the unusual position of a complementary sequence to 18S rRNA in eEF1A mRNA. Amino acids. 47(2):345-56. 2015. Su H, Meng S, Lu Y. Mammalian hyperplastic discs homolog EDD regulates miRNA-mediated gene silencing. Molecular cell. 43(1):97-109. 2011.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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Author: Gardos- Channel