Name :
GUF1 antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
GUF1 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, HepG2 cells, mouse brain tissue, mouse liver tissue, mouse pancreas tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human oesophagus tissue, human kidney tissue, human lung cancer tissue, human testis tissue. Observed molecular weight by Western-blot: 70 kDa
Tested applications :
ELISA, WB, IF, IHC, IP
Species reactivity :
Human, Mouse; other species not tested.
Alternative names :
FLJ13220 antibody; GUF1 antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of GUF1 recombinant protein (Accession Number: NM_021927). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:500-1:5000IP: 1:200-1:2000IHC: 1:20-1:200IF: 1:10-1:100
Background :
GUF1 (GUF1 GTPase), also known as EF-4 (elongation factor 4 homolog), is a 669 amino acid protein belonging to the GTP-binding elongation factor family and the LepA subfamily. Localizing to the mitochondrion inner membrane, GUF1 promotes mitochondrial protein synthesis and binds to mitochondrial ribosomes in a GTP-dependent manner. GUF1 may act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. In western blotting, we got double bands 74 kDa and 70 kDa, due to the N-terminal 1-49 amino acid sequence that could be cut down.
References :
Related websites: https://www.medchemexpress.com/antibodies.html
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