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Name :
H3F3B antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
H3F3B Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells, HepG2 cells. Observed molecular weight by Western-blot: 17 kDa

Tested applications :

Species reactivity :
Human, Mouse, Rat; other species not tested.

Alternative names :
H3 histone antibody; family 3B (H3.3B) antibody; H3.3A antibody; H3.3B antibody; H3F3 antibody; H3F3A antibody; H3F3B antibody; Histone H3.3 antibody

Immunogen :
Human H3F3B Recombinant protein (GST tag)

Isotype :
Rabbit IgG

Preparation :
This antibody was obtained by immunization of H3F3B recombinant protein (Accession Number: NM_001379045). Purification method: Antigen affinity purified.

Clonality :

Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:200-1:1000

Background :
Histones are the basic nuclear proteins responsible for the nucleosome structure of the chromosomal fiber. Five classes of histone genes have been reported, some of which are expressed only during S phase, while others are replication independent. The latter are referred to as replacement histones and are expressed in quiescent or terminally differentiated cells. H3F3B is one replacement histone [PMID: 8586426]. It constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Thereby it has a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability [PMID: 14718166, 15776021].

References :

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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Author: Gardos- Channel