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Name :
HA-Tag antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
HA-Tag Mouse Monoclonal antibody. Positive IF detected in Transfected HEK-293 cells. Positive IP detected in Transfected HEK-293 cells. Positive WB detected in Recombinant protein.

Tested applications :

Species reactivity :
Recombinant protein; other species not tested.

Alternative names :
HA Tag antibody; YPYDVPDYA antibody

Immunogen :

Isotype :
Mouse IgG1

Preparation :
This antibody was obtained by immunization of Peptide (Accession Number: HA). Purification method: Protein A purified.

Clonality :

Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:5000-1:50000IP: 1:2000-1:20000IF: 1:10-1:100

Background :
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.

References :
Lu B, Sun X, Chen Y. Novel function of PITH domain-containing 1 as an activator of internal ribosomal entry site to enhance RUNX1 expression and promote megakaryocyte differentiation. Cellular and molecular life sciences : CMLS. 72(4):821-32. 2015. Zhao N, Gong P, Cheng B. Eimeria tenella: 14-3-3 protein interacts with telomerase. Parasitology research. 113(10):3885-9. 2014. Xiong W, Yang J, Wang M. Vinexin β Interacts with Hepatitis C Virus NS5A, Modulating Its Hyperphosphorylation To Regulate Viral Propagation. Journal of virology. 89(14):7385-400. 2015. Tsai YC, Lapina MC, Bhushan S, Mueller-Cajar O. Identification and characterization of multiple rubisco activases in chemoautotrophic bacteria. Nature communications. 6:8883. 2015.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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Author: Gardos- Channel