Name :
Hemoglobin epsilon antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
Hemoglobin epsilon Rabbit Polyclonal antibody. Positive FC detected in K-562 cells. Positive IHC detected in human placenta tissue. Positive WB detected in human placenta tissue, K-562 cells, mouse placenta tissue. Observed molecular weight by Western-blot: 12-16 kDa
Tested applications :
ELISA, WB, IHC, FC
Species reactivity :
Human,Mouse,Rat; other species not tested.
Alternative names :
Epsilon globin antibody; HBE antibody; HBE1 antibody; Hemoglobin epsilon chain antibody; Hemoglobin subunit epsilon antibody; hemoglobin antibody; epsilon 1 antibody
Immunogen :
Human HBE1 Recombinant protein (GST tag)
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of Hemoglobin epsilon recombinant protein (Accession Number: NM_005330). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:500-1:5000IHC: 1:20-1:200
Background :
The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBE1 (hemoglobin epsilon chain) is a beta-type chain of hemoglobin predominantly expressed during the embryonic stage (21321359). The HBE1 has been regarded as the best marker for fetal nucleated red blood cells (NRBCs) . This antibody can be used to label and isolate fetal cells from maternal blood and may be useful in prenatal diagnosis (15906407).
References :
Wei L, Jia L, Zhu L. A comparison of E15.5 fetus and newborn rat serum proteomes. Proteome science. 10(1):64. 2012. Ma Y, Wang B, Jiang F. A feedback loop consisting of microRNA 23a/27a and the β-like globin suppressors KLF3 and SP1 regulates globin gene expression. Molecular and cellular biology. 33(20):3994-4007. 2013. Starnes LM, Sorrentino A, Pelosi E. NFI-A directs the fate of hematopoietic progenitors to the erythroid or granulocytic lineage and controls beta-globin and G-CSF receptor expression. Blood. 114(9):1753-63. 2009. Vian L, Di Carlo M, Pelosi E. Transcriptional fine-tuning of microRNA-223 levels directs lineage choice of human hematopoietic progenitors. Cell death and differentiation. 21(2):290-301. 2014.
Related websites: https://www.medchemexpress.com/antibodies.html
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