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Name :
HINT1 antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
HINT1 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive WB detected in HEK-293 cells, K-562 cells, Raji cells, rat brain tissue, rat thymus tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 14 kDa

Tested applications :

Species reactivity :
Human,Mouse,Rat; other species not tested.

Alternative names :
FLJ30414 antibody; FLJ32340 antibody; HINT antibody; HINT1 antibody; PKCI 1 antibody; PKCI1 antibody; PRKCNH1 antibody; Protein kinase C inhibitor 1 antibody

Immunogen :
Human HINT1 Recombinant protein (GST tag)

Isotype :
Rabbit IgG

Preparation :
This antibody was obtained by immunization of HINT1 recombinant protein (Accession Number: NM_005340). Purification method: Antigen affinity purified.

Clonality :

Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:500-1:5000IP: 1:200-1:2000IHC: 1:20-1:200

Background :
HINT1(Histidine triad nucleotide-binding protein 1) is also named as HINT, PKCI1, PRKCNH1,which is a member of the histidine triad (HIT) family, highly conserved in diverse species and ubiquitously expressed in mammalian tissues.It hydrolyzes adenosine 5′-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.This antibody is specific to HINT1.

References :
Cen B, Li H, Weinstein IB. Histidine triad nucleotide-binding protein 1 up-regulates cellular levels of p27KIP1 by targeting ScfSKP2 ubiquitin ligase and Src. The Journal of biological chemistry. 284(8):5265-76. 2009. Ho J, Kong JW, Choong LY. Novel breast cancer metastasis-associated proteins. Journal of proteome research. 8(2):583-94. 2009. Berglund SR, Santana AR, Li D, Rice RH, Rocke DM, Goldberg Z. Proteomic analysis of low dose arsenic and ionizing radiation exposure on keratinocytes. Proteomics. 9(7):1925-38. 2009. Zhang YJ, Li H, Wu HC. Silencing of Hint1, a novel tumor suppressor gene, by promoter hypermethylation in hepatocellular carcinoma. Cancer letters. 275(2):277-84. 2009. Hsieh SY, Hsu CY, He JR. Identifying apoptosis-evasion proteins/pathways in human hepatoma cells via induction of cellular hormesis by UV irradiation. Journal of proteome research. 8(8):3977-86. 2009. Jackson KJ, Chen Q, Chen J, Aggen SH, Kendler KS, Chen X. Association of the histidine-triad nucleotide-binding protein-1 (HINT1) gene variants with nicotine dependence. The pharmacogenomics journal. 11(4):251-7. 2011. Murakami E, Tolstykh T, Bao H. Mechanism of activation of PSI-7851 and its diastereoisomer PSI-7977. The Journal of biological chemistry. 285(45):34337-47. 2010. Genovese G, Ghosh P, Li H. The tumor suppressor HINT1 regulates MITF and β-catenin transcriptional activity in melanoma cells. Cell cycle (Georgetown, Tex.). 11(11):2206-15. 2012.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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Author: Gardos- Channel