Name :
HINT1 antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
HINT1 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive WB detected in HEK-293 cells, K-562 cells, Raji cells, rat brain tissue, rat thymus tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 14 kDa
Tested applications :
ELISA, WB, IHC, IP
Species reactivity :
Human,Mouse,Rat; other species not tested.
Alternative names :
FLJ30414 antibody; FLJ32340 antibody; HINT antibody; HINT1 antibody; PKCI 1 antibody; PKCI1 antibody; PRKCNH1 antibody; Protein kinase C inhibitor 1 antibody
Immunogen :
Human HINT1 Recombinant protein (GST tag)
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of HINT1 recombinant protein (Accession Number: NM_005340). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:500-1:5000IP: 1:200-1:2000IHC: 1:20-1:200
Background :
HINT1(Histidine triad nucleotide-binding protein 1) is also named as HINT, PKCI1, PRKCNH1,which is a member of the histidine triad (HIT) family, highly conserved in diverse species and ubiquitously expressed in mammalian tissues.It hydrolyzes adenosine 5′-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.This antibody is specific to HINT1.
References :
Cen B, Li H, Weinstein IB. Histidine triad nucleotide-binding protein 1 up-regulates cellular levels of p27KIP1 by targeting ScfSKP2 ubiquitin ligase and Src. The Journal of biological chemistry. 284(8):5265-76. 2009. Ho J, Kong JW, Choong LY. Novel breast cancer metastasis-associated proteins. Journal of proteome research. 8(2):583-94. 2009. Berglund SR, Santana AR, Li D, Rice RH, Rocke DM, Goldberg Z. Proteomic analysis of low dose arsenic and ionizing radiation exposure on keratinocytes. Proteomics. 9(7):1925-38. 2009. Zhang YJ, Li H, Wu HC. Silencing of Hint1, a novel tumor suppressor gene, by promoter hypermethylation in hepatocellular carcinoma. Cancer letters. 275(2):277-84. 2009. Hsieh SY, Hsu CY, He JR. Identifying apoptosis-evasion proteins/pathways in human hepatoma cells via induction of cellular hormesis by UV irradiation. Journal of proteome research. 8(8):3977-86. 2009. Jackson KJ, Chen Q, Chen J, Aggen SH, Kendler KS, Chen X. Association of the histidine-triad nucleotide-binding protein-1 (HINT1) gene variants with nicotine dependence. The pharmacogenomics journal. 11(4):251-7. 2011. Murakami E, Tolstykh T, Bao H. Mechanism of activation of PSI-7851 and its diastereoisomer PSI-7977. The Journal of biological chemistry. 285(45):34337-47. 2010. Genovese G, Ghosh P, Li H. The tumor suppressor HINT1 regulates MITF and β-catenin transcriptional activity in melanoma cells. Cell cycle (Georgetown, Tex.). 11(11):2206-15. 2012.
Related websites: https://www.medchemexpress.com/antibodies.html
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