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Name :
Histone H2A.X antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
Histone H2A.X Rabbit Polyclonal antibody. Positive FC detected in HepG2 cells. Positive IHC detected in human lymphoma tissue. Positive IF detected in HepG2 cells. Positive WB detected in Raji cells, HEK-293 cells, HL-60 cells, mouse heart tissue. Observed molecular weight by Western-blot: 15-18kd

Tested applications :

Species reactivity :
Human,Mouse,Rat; other species not tested.

Alternative names :
H2A histone family antibody; member X antibody; H2A.X antibody; H2A/X antibody; H2AFX antibody; H2AX antibody; Histone H2A.x antibody

Immunogen :

Isotype :
Rabbit IgG

Preparation :
This antibody was obtained by immunization of Histone H2A.X recombinant protein (Accession Number: NM_002105). Purification method: Antigen affinity purified.

Clonality :

Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:200-1:2000IHC: 1:20-1:200IF: 1:10-1:100

Background :
Histone H2A.X belongs to the histone H2A family, which is synthesized in G1 and S phase. It is involved in nucleosomal organization of chromatin together with other histone proteins, and is specially important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, which promotes DNA repair and maintains genomic stability.

References :
Dickey JS, Redon CE, Nakamura AJ, Baird BJ, Sedelnikova OA, Bonner WM. H2AX: functional roles and potential applications. Chromosoma. 118(6):683-92. 2009. Wu Y, Sun L, Zou W. Prosaposin, a regulator of estrogen receptor alpha, promotes breast cancer growth. Cancer science. 103(10):1820-5. 2012. Chen X, Zhang Z, Feng Y. Aberrant survivin expression in endometrial hyperplasia: another mechanism of progestin resistance. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc. 22(5):699-708. 2009. Wang Y, Huang Y, Guan F. Hypoxia-inducible factor-1alpha and MAPK co-regulate activation of hepatic stellate cells upon hypoxia stimulation. PloS one. 8(9):e74051. 2013. Qi W, Wang R, Chen H. BRG1 promotes the repair of DNA double-strand breaks by facilitating the replacement of RPA with RAD51. Journal of cell science. 128(2):317-30. 2015. Yaglom JA, McFarland C, Mirny L, Sherman MY. Oncogene-triggered suppression of DNA repair leads to DNA instability in cancer. Oncotarget. 5(18):8367-78. 2014. Wu P, Zhu X, Jin W, Hao S, Liu Q, Zhang L. Oxaliplatin triggers necrosis as well as apoptosis in gastric cancer SGC-7901 cells. Biochemical and biophysical research communications. 460(2):183-90. 2015. Xu Y, Wu D, Fan Y. Novel Recombinant Protein FlaA N/C Protects against Radiation Injury via NF-κB Signaling. Radiation research. 185(1):77-86. 2016.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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Author: Gardos- Channel