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Name :
HLA class I (HLA-A) antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
HLA class I (HLA-A) Mouse Monoclonal antibody. Positive WB detected in Hepg2 cells, HeLa cells, Raji cells, U-937 cells. Positive IHC detected in human tonsillitis tissue, human heart tissue, human spleen tissue. Positive FC detected in HepG2 cells, HEK-293 cells. Observed molecular weight by Western-blot: 44 kDa

Tested applications :

Species reactivity :
Human, Mouse, Rat, Pig; other species not tested.

Alternative names :
HLA A antibody; HLA class I antibody; HLA class I (HLA-A) antibody; HLAA antibody; HLA-A antibody; MHC class I antigen A*3 antibody

Immunogen :

Isotype :
Mouse IgG2b

Preparation :
This antibody was obtained by immunization of HLA class I (HLA-A) recombinant protein (Accession Number: NM_001242758). Purification method: Protein A purified.

Clonality :

Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:200-1:2000IHC: 1:20-1:200

Background :
Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)

References :
Zhao X, Sun Q, Tian H, Cong B, Jiang X, Peng C. Loss of heterozygosity at 6p21 and HLA class I expression in esophageal squamous cell carcinomas in China. Asian Pacific journal of cancer prevention : APJCP. 12(10):2741-5. 2011.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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Author: Gardos- Channel