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Name :
HNRNPF antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
HNRNPF Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human colon cancer tissue. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 46 kDa

Tested applications :

Species reactivity :
Human,Mouse,Rat; other species not tested.

Alternative names :
hnRNP F antibody; HNRNPF antibody; HNRPF antibody; mcs94 1 antibody; Nucleolin like protein mcs94 1 antibody; OK/SW cl.23 antibody

Immunogen :

Isotype :
Rabbit IgG

Preparation :
This antibody was obtained by immunization of HNRNPF recombinant protein (Accession Number: NM_001098204). Purification method: Antigen affinity purified.

Clonality :

Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:500-1:5000IHC: 1:20-1:200IF: 1:50-1:500

Background :
HNRNPF, also named as Heterogeneous nuclear ribonucleoprotein F,is a 415 amino acid portein, which contains 3 RRM (RNA recognition motif) domains. HNRNPF is expressed ubiquitously in various tissues and localizes in the nucleus. HNRNPF is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPF plays a role in the regulation of alternative splicing events.

References :
Su CC, Su JH, Lin JJ. An investigation into the cytotoxic effects of 13-acetoxysarcocrassolide from the soft coral Sarcophyton crassocaule on bladder cancer cells. Marine drugs. 9(12):2622-42. 2011.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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Author: Gardos- Channel