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Name :
HSD11B2 antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
HSD11B2 Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue. Positive WB detected in human kidney tissue, human placenta tissue, mouse kidney tissue, Transfected HEK-293 cells. Observed molecular weight by Western-blot: 40-43 kDa

Tested applications :

Species reactivity :
Human,Mouse,Rat; other species not tested.

Alternative names :
11 beta HSD2 antibody; 11 DH2 antibody; AME antibody; AME1 antibody; HSD11B2 antibody; HSD11K antibody; HSD2 antibody; SDR9C3 antibody

Immunogen :

Isotype :
Rabbit IgG

Preparation :
This antibody was obtained by immunization of HSD11B2 recombinant protein (Accession Number: NM_000196). Purification method: Antigen affinity purified.

Clonality :

Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:200-1:1000IHC: 1:20-1:200

Background :
The HSD11B2 gene encodes the type II isoform of 11-beta-hydroxysteroid dehydrogenase, a microsomal enzyme complex responsible for the interconversion of biologically active cortisol and inactive cortisone. This protein catalyzes the cortisol-to-cortisone reaction. This protein expresses approximately 44 kDa in placenta, trophoblast, and distal colon, but in kidney tissue, there are two bands of approximately 44 and 48 kDa can be consistently observed and no signal is seen in decidua, adrenal, or liver(PMID:9048640). HSD11B2 protein was detected in CEM-C7 cells via immunoblot analysis as a dimer and trimer of approximately 80 and 120 kDa (PMID:23762608).

References :
Moravec M, Svec J, Ergang P. Expression of 11β-hydroxysteroid dehydrogenase type 2 is deregulated in colon carcinoma. Histology and histopathology. 29(4):489-96. 2014. Tetsi Nomigni M, Ouzounian S, Benoit A. Steroidogenic enzyme profile in an androgen-secreting adrenocortical oncocytoma associated with hirsustism. Endocrine connections. 4(2):117-27. 2015.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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Author: Gardos- Channel