Name :
KGA/GAC antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
KGA/GAC Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, mouse brain tissue, mouse kidney tissue. Positive IP detected in mouse brain tissue. Positive IHC detected in human gliomas tissue, human brain tissue, human kidney tissue. Observed molecular weight by Western-blot: 65kd,58kd
Tested applications :
ELISA, IHC, IP, WB
Species reactivity :
Human,Mouse,Rat; other species not tested.
Alternative names :
AAD20 antibody; DKFZp686O15119 antibody; FLJ10358 antibody; GAC antibody; GLS antibody; GLS1 antibody; glutaminase antibody; K glutaminase antibody; KGA antibody; KIAA0838 antibody; L glutamine amidohydrolase antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of KGA/GAC recombinant protein (Accession Number: NM_014905). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:200-1:2000IP: 1:200-1:2000IHC: 1:20-1:200
Background :
GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer’s disease(PMID:1672808).The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody can recognize KGA and GAC.
References :
Colombo SL, Palacios-Callender M, Frakich N. Anaphase-promoting complex/cyclosome-Cdh1 coordinates glycolysis and glutaminolysis with transition to S phase in human T lymphocytes. Proceedings of the National Academy of Sciences of the United States of America. 107(44):18868-73. 2010. Shimmura C, Suzuki K, Iwata Y. Enzymes in the glutamate-glutamine cycle in the anterior cingulate cortex in postmortem brain of subjects with autism. Molecular autism. 4(1):6. 2013. Xu FF, Huang Y, Wang XQ, Qiu YH, Peng YP. Modulation of immune function by glutamatergic neurons in the cerebellar interposed nucleus via hypothalamic and sympathetic pathways. Brain, behavior, and immunity. 38:263-71. 2014. Masamha CP, Xia Z, Yang J. CFIm25 links alternative polyadenylation to glioblastoma tumour suppression. Nature. 510(7505):412-6. 2014. Yeo H, Lyssiotis CA, Zhang Y. FoxO3 coordinates metabolic pathways to maintain redox balance in neural stem cells. The EMBO journal. 32(19):2589-602. 2013. Oricchio E, Papapetrou EP, Lafaille F. A cell engineering strategy to enhance the safety of stem cell therapies. Cell reports. 8(6):1677-85. 2014. Yang HW, Kim TM, Song SS. A small subunit processome protein promotes cancer by altering translation. Oncogene. 34(34):4471-81. 2015. Karner CM, Esen E, Okunade AL, Patterson BW, Long F. Increased glutamine catabolism mediates bone anabolism in response to WNT signaling. The Journal of clinical investigation. 125(2):551-62. 2015.
Related websites: https://www.medchemexpress.com/antibodies.html
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