Name :
KGA/GAM/GAC antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
KGA/GAM/GAC Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, HeLa cells, mouse brain tissue, mouse liver tissue, rat brain tissue. Positive IP detected in HEK-293 cells. Positive IHC detected in human kidney tissue, human brain tissue. Positive IF detected in HEK-293 cells, HeLa cells. Observed molecular weight by Western-blot: 58 kDa, 83 kDa
Tested applications :
ELISA, IHC, IF, WB, IP
Species reactivity :
Human, Mouse, Rat; other species not tested.
Alternative names :
AAD20 antibody; DKFZp686O15119 antibody; FLJ10358 antibody; GAC antibody; GAM antibody; GLS antibody; GLS1 antibody; glutaminase antibody; K glutaminase antibody; KGA antibody; KIAA0838 antibody; L glutamine amidohydrolase antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of KGA/GAM/GAC recombinant protein (Accession Number: NM_014905). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:200-1:2000IP: 1:500-1:5000IHC: 1:20-1:200IF: 1:10-1:100
Background :
GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer’s disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa(PMID:12408749). Ping Gao et al. (2009) determined that mitochondrial glutaminase expression (GLS, molecular mass of ~58 kDa) is increased ~10-fold in response to Myc. It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). GLS has 3 isoforms produced by alternative splicing and this antibody is specific to the 3 isoforms(KGA,GAM,GAC) of GLS.
References :
Smith MR, Vayalil PK, Zhou F. Mitochondrial thiol modification by a targeted electrophile inhibits metabolism in breast adenocarcinoma cells by inhibiting enzyme activity and protein levels. Redox biology. 8:136-148. 2016.
Related websites: https://www.medchemexpress.com/antibodies.html
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