Name :
KGA-Specific antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
KGA-Specific Rabbit Polyclonal antibody. Positive WB detected in HEK-293 cells, human brain tissue, human heart tissue, K-562 cells, mouse brain tissue, mouse kidney tissue. Positive IP detected in HEK-293 cells. Positive IHC detected in human kidney tissue, human brain tissue. Observed molecular weight by Western-blot: 66 kDa
Tested applications :
ELISA, IHC, WB, IP
Species reactivity :
Human,Mouse,Rat; other species not tested.
Alternative names :
AAD20 antibody; DKFZp686O15119 antibody; FLJ10358 antibody; GLS antibody; GLS1 antibody; glutaminase antibody; K glutaminase antibody; KGA antibody; KGA-specific antibody; KIAA0838 antibody; L glutamine amidohydrolase antibody
Immunogen :
Human GLS Recombinant protein (GST tag)
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of KGA-Specific recombinant protein (Accession Number: NM_014905). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:200-1:2000IP: 1:200-1:2000IHC: 1:20-1:200
Background :
GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer’s disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms produced by alternative splicing named as KGA,GAM,GAC. This antibody is specific to KGA.
References :
Jacque N, Ronchetti AM, Larrue C. Targeting glutaminolysis has antileukemic activity in acute myeloid leukemia and synergizes with BCL-2 inhibition. Blood. 126(11):1346-56. 2015. Herranz D, Ambesi-Impiombato A, Sudderth J. Metabolic reprogramming induces resistance to anti-NOTCH1 therapies in T cell acute lymphoblastic leukemia. Nature medicine. 21(10):1182-9. 2015.
Related websites: https://www.medchemexpress.com/antibodies.html
Popular product recommendations:
Galcanezumab
Cy5-conjugated AffiniPure Goat Anti-Rabbit IgG H&L
Phospho-Smad3 (Ser423/425) Antibody: Phospho-Smad3 (Ser423/425) Antibody is a non-conjugated and Rabbit origined polyclonal antibody about 48 kDa, targeting to Phospho-Smad3 (Ser423/425). It can be used for WB,IHC-P,ICC/IF assays with tag free, in the background of Human, Mouse.
