Name :
Mitofilin antibody
Documents :
DataSheet Material Safety Data Sheets (MSDS)
Description :
Mitofilin Rabbit Polyclonal antibody. Positive FC detected in HEK-293T cells. Positive IHC detected in human gliomas tissue, human breast cancer tissue. Positive IF detected in HepG2 cells. Positive WB detected in multi-cells/tissue, COLO 320 cells, HEK-293 cells, HeLa cells, HepG2 cells, human brain tissue, MCF7 cells, Raji cells. Positive IP detected in Raji cells. Observed molecular weight by Western-blot: 87 kDa,89 kDa
Tested applications :
ELISA, IHC, IF, WB, IP, FC
Species reactivity :
Human, Mouse; other species not tested.
Alternative names :
DKFZp779P1653 antibody; HMP antibody; IMMT antibody; MIC60 antibody; MINOS2 antibody; Mitofilin antibody; P87 antibody; P87/89 antibody; P89 antibody; PIG4 antibody; PIG52 antibody
Immunogen :
Isotype :
Rabbit IgG
Preparation :
This antibody was obtained by immunization of Mitofilin recombinant protein (Accession Number: XM_017003194). Purification method: Antigen affinity purified.
Clonality :
Polyclonal
Formulation :
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage instructions :
Store at -20℃. DO NOT ALIQUOT
Applications :
Recommended Dilution: WB: 1:500-1:5000IP: 1:500-1:5000IHC: 1:20-1:200IF: 1:20-1:200
Background :
IMMT (also known as mitofilin) is an inner mitochondrial membrane protein that is preferentially expressed in heart tissue and is commonly used as the marker for mitochondria. Mitofilin is known to be a critical organizer of mitochondrial cristae morphology and is indispensable for normal mitochondrial function. Three isoforms of mitofilin exist due to the alternative splicing. All three proteins of 87kD, 89kD and 80kD can be detected in immunoblot analysis with this antibody.
References :
Ding C, Wu Z, Huang L. Mitofilin and CHCHD6 physically interact with Sam50 to sustain cristae structure. Scientific reports. 5:16064. 2015. Genin EC, Plutino M, Bannwarth S. CHCHD10 mutations promote loss of mitochondrial cristae junctions with impaired mitochondrial genome maintenance and inhibition of apoptosis. EMBO molecular medicine. 8(1):58-72. 2015. Mateo R, Nagamine CM, Kirkegaard K. Suppression of Drug Resistance in Dengue Virus. mBio. 6(6):e01960-15. 2015. Yin X, Mayr M, Xiao Q, Wang W, Xu Q. Proteomic analysis reveals higher demand for antioxidant protection in embryonic stem cell-derived smooth muscle cells. Proteomics. 6(24):6437-46. 2006. Weihofen A, Thomas KJ, Ostaszewski BL, Cookson MR, Selkoe DJ. Pink1 forms a multiprotein complex with Miro and Milton, linking Pink1 function to mitochondrial trafficking. Biochemistry. 48(9):2045-52. 2009. Jiang L, Fang J, Moore DS. Age-associated changes in synaptic lipid raft proteins revealed by two-dimensional fluorescence difference gel electrophoresis. Neurobiology of aging. 31(12):2146-59. 2010. An J, Shi J, He Q. CHCM1/CHCHD6, novel mitochondrial protein linked to regulation of mitofilin and mitochondrial cristae morphology. The Journal of biological chemistry. 287(10):7411-26. 2012. Anand R, Wai T, Baker MJ. The i-AAA protease YME1L and OMA1 cleave OPA1 to balance mitochondrial fusion and fission. The Journal of cell biology. 204(6):919-29. 2014.
Related websites: https://www.medchemexpress.com/antibodies.html
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