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Name :
HEPACAM antibody

Documents :
DataSheet Material Safety Data Sheets (MSDS)

Description :
HEPACAM Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, HepG2 cells, MCF7 cells, rat brain tissue, SH-SY5Y cells. Positive IP detected in rat brain tissue. Positive IF detected in MCF-7 cells. Positive IHC detected in human liver tissue. Observed molecular weight by Western-blot: 46-72 kDa

Tested applications :

Species reactivity :
Human,Mouse,Rat; other species not tested.

Alternative names :
FLJ25530 antibody; GlialCAM antibody; HEPACAM antibody; Protein hepaCAM antibody

Immunogen :

Isotype :
Rabbit IgG

Preparation :
This antibody was obtained by immunization of HEPACAM recombinant protein (Accession Number: NM_152722). Purification method: Antigen affinity purified.

Clonality :

Formulation :
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions :
Store at -20℃. DO NOT ALIQUOT

Applications :
Recommended Dilution: WB: 1:200-1:2000IP: 1:200-1:2000IHC: 1:20-1:200IF: 1:10-1:100

Background :
HepaCAM (hepatocyte cell adhesion molecule), also known as GlialCAM, is a single-pass type I membrane glycoprotein of 416 amino acids. It displays a typical structure of immunoglobulin (Ig)-like adhesion molecules including two extracellular Ig-like domains, a transmembrane segment, and a cytoplasmic tail. It has been shown that hepaCAM forms a cis-homodimer on the cell surface, and modulates cell-matrix interaction. It is predominantly expressed in the CNS glial cells. Defects in HEPACAM gene are the cause of megalencephalic leukoencephalopathy with subcortical cysts type 2A (MLC2A). HEPACAM has also been suggested as a tumor suppressor gene.

References :
Lanciotti A, Brignone MS, Molinari P. Megalencephalic leukoencephalopathy with subcortical cysts protein 1 functionally cooperates with the TRPV4 cation channel to activate the response of astrocytes to osmotic stress: dysregulation by pathological mutations. Human molecular genetics. 21(10):2166-80. 2012. Xu B, He Y, Wu X, Luo C, Liu A, Zhang J. Exploration of the correlations between interferon-γ in patient serum and HEPACAM in bladder transitional cell carcinoma, and the interferon-γ mechanism inhibiting BIU-87 proliferation. The Journal of urology. 188(4):1346-53. 2012. Tao J, Liu Q, Wu X. Identification of hypermethylation in hepatocyte cell adhesion molecule gene promoter region in bladder carcinoma. International journal of medical sciences. 10(13):1860-7. 2013. Tan B, Tan J, Du H. HepaCAM inhibits clear cell renal carcinoma 786-0 cell proliferation via blocking PKCε translocation from cytoplasm to plasma membrane. Molecular and cellular biochemistry. 391(1-2):95-102. 2014. Du HF, Ou LP, Lv CK. Expression of hepaCAM inhibits bladder cancer cell proliferation via a Wnt/β-catenin-dependent pathway in vitro and in vivo. Cancer biology & therapy. 16(10):1502-13. 2015. Wang X, Chen E, Tang M. The SMAD2/3 pathway is involved in hepaCAM-induced apoptosis by inhibiting the nuclear translocation of SMAD2/3 in bladder cancer cells. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 2016.

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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Author: Gardos- Channel